Signal enhancement on gold nanoparticle-based lateral flow tests using cellulose nanofibers
Quesada-González, Daniel (Institut Català de Nanociència i Nanotecnologia)
Stefani, Christina (Institut Català de Nanociència i Nanotecnologia)
González Tovar, Israel (Universitat de Girona)
De La Escosura-Muñiz, Alfredo (Institut Català de Nanociència i Nanotecnologia)
Domingo Marimon, Neus (Institut Català de Nanociència i Nanotecnologia)
Mutjé, Pere (Universitat de Girona)
Merkoçi, Arben (Institut Català de Nanociència i Nanotecnologia)

Date:	2019
Abstract:	Lateral flow paper-based biosensors merge as powerful tools in point-of-care diagnostics since they are cheap, portable, robust, selective, fast and easy to use. However, the sensitivity of this type of biosensors is not always as high as required, often not permitting a clear quantification. To improve the colorimetric response of standard lateral flow strips (LFs), we have applied a new enhancement strategy that increases the sensitivity of LFs based on the use of cellulose nanofibers (CNF). CNF penetrate inside the pores of LFs nitrocellulose paper, compacting the pore size only in the test line, particularly near the surface of the strip. This modification retains the bioreceptors (antibodies) close to the surface of the strips, and thus further increasing the density of selectively attached gold nanoparticles (AuNPs) in the top part of the membrane, in the test line area, only when the sample is positive. This effect boosts in average a 36. 6% the sensitivity of the LFs. The optical measurements of the LFs were carried out with a mobile phone camera whose imaging resolution was improved by attaching microscopic lens on the camera objective. The characterization of CNF into paper and their effect was analyzed using atomic force microscope (AFM) and scanning electron microscope (SEM) imaging techniques.
Grants:	Ministerio de Economía y Competitividad SEV-2017-0706 Ministerio de Economía y Competitividad MAT2017-87202-P Ministerio de Economía y Competitividad FIS2015-73932-JIN Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-579 European Commission 754558
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	Lateral flow ; Biosensor ; Cellulose ; Gold nanoparticles ; Nanofibers
Published in:	Biosensors & bioelectronics, Vol. 141 (September 2019) , art. 111407, ISSN 1873-4235

DOI: 10.1016/j.bios.2019.111407

Postprint 9 p, 524.5 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles