Validity of a single antibody-based lateral flow immunoassay depending on graphene oxide for highly sensitive determination of E. coli O157:H7 in minced beef and river water
Hassan, Abdel-Raim H. A. ![ORCID Identifier](/img/uab/orcid.ico)
(Institut Català de Nanociència i Nanotecnologia)
Bergua Canudo, José Francisco ![ORCID Identifier](/img/uab/orcid.ico)
(Institut Català de Nanociència i Nanotecnologia)
Morales-Narváez, Eden ![ORCID Identifier](/img/uab/orcid.ico)
(Biophotonic Nanosensors Laboratory)
Merkoçi, Arben ![ORCID Identifier](/img/uab/orcid.ico)
(Institut Català de Nanociència i Nanotecnologia)
Date: |
2019 |
Abstract: |
Considering the health risks of E. coli O157:H7 presence in food and water, an affordable and highly sensitive detection method is crucial. Herein, we report the first use of a single antibody-based fluorescent lateral flow immunoassay (FLFIA) depending on non-radiative energy transfer between graphene oxide and quantum dots for determination of E. coli O157:H7 in beef and river water. FLFIA showed a high sensitivity rate thousand-fold better than the conventional lateral flow (LF). In inoculated minced beef and river water samples, the limits of detection were 178 and 133 CFU g or mL, respectively. Besides, it presented a high selectivity in the presence of other possible interfering bacteria. The single antibody approach reduced the assay cost to 60% less than the conventional LF. Alongside, the results could be read by portable LF readers or smartphones. These advantages offer FLFIA as a promising technology for pathogen detection in food and water. |
Grants: |
Ministerio de Economía y Competitividad SEV-2017-0706
|
Rights: |
Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. ![Creative Commons](/img/licenses/by-nc-nd.ico) |
Language: |
Anglès |
Document: |
Article ; recerca ; Versió acceptada per publicar |
Subject: |
Graphene oxide ;
Fluorescent lateral flow ;
E. coli O157:H7 ;
Food safety ;
Minced beef ;
Water quality |
Published in: |
Food chemistry, Vol. 297 (November 2019) , art. 124965, ISSN 1873-7072 |
DOI: 10.1016/j.foodchem.2019.124965
The record appears in these collections:
Research literature >
UAB research groups literature >
Research Centres and Groups (research output) >
Experimental sciences >
Catalan Institute of Nanoscience and Nanotechnology (ICN2)Articles >
Research articlesArticles >
Published articles
Record created 2020-02-06, last modified 2023-10-01