Label-free Bacteria Quantification in Blood Plasma by a Bioprinted Microarray Based Interferometric Point-of-Care Device
Dey, Priyanka (Institut Català de Nanociència i Nanotecnologia)
Fabri Faja, Nuria (Institut Català de Nanociència i Nanotecnologia)
Calvo Lozano, Olalla (Institut Català de Nanociència i Nanotecnologia)
Terborg, Roland A. (Institut de Ciències Fotòniques)
Belushkin, Alexander (École Polytechnique Fédérale de Lausanne)
Yesilköy, Filiz (École Polytechnique Fédérale de Lausanne)
Fàbrega-Santamaria, Anna (Hospital Universitari Vall d'Hebron)
Ruiz Rodríguez, Juan Carlos (Hospital Universitari Vall d'Hebron)
Ferrer, Ricard (Hospital Universitari Vall d'Hebron)
González-López, Juanjo 1975- (Hospital Universitari Vall d'Hebron)
Estévez, M. Carmen (Institut Català de Nanociència i Nanotecnologia)
Altug, Hatice (École Polytechnique Fédérale de Lausanne)
Pruneri, Valerio (Institució Catalana de Recerca i Estudis Avançats)
Lechuga, Laura M (Institut Català de Nanociència i Nanotecnologia)

Date:	2019
Abstract:	Existing clinical methods for bacteria detection lack speed, sensitivity, and, importantly, point-of-care (PoC) applicability. Thus, finding ways to push the sensitivity of clinical PoC biosensing technologies is crucial. Here we report a portable PoC device based on lens-free interferometric microscopy (LIM). The device employs high performance nanoplasmonics and custom bioprinted microarrays and is capable of direct label-free bacteria (E. coli) quantification. With only one-step sample handling we offer a sample-to-data turnaround time of 40 min. Our technology features detection sensitivity of a single bacterial cell both in buffer and in diluted blood plasma and is intrinsically limited by the number of cells present in the detection volume. When employed in a hospital setting, the device has enabled accurate categorization of sepsis patients (infectious SIRS) from control groups (healthy individuals and noninfectious SIRS patients) without false positives/negatives. User-friendly on-site bacterial clinical diagnosis can thus become a reality.
Grants:	European Commission 644956 Ministerio de Economía y Competitividad SEV-2013-0295 Ministerio de Economía y Competitividad FICTS-1420-27 Ministerio de Economía y Competitividad SEV-2015-0522 Ministerio de Economía y Competitividad TEC2016-75080-R Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-1634
Rights:	Tots els drets reservats.
Language:	Anglès
Document:	Article ; recerca ; Versió sotmesa a revisió
Subject:	Bacteria ; Sepsis ; Microarray ; Label-free detection ; Nanoplasmonics ; Plasma samples
Published in:	ACS Sensors, Vol. 4, Issue 1 (January 2019) , p. 52-60, ISSN 2379-3694

DOI: 10.1021/acssensors.8b00789
PMID: 30525470

Preprint 24 p, 1.4 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles