Practical applications of sperm selection techniques for improving reproductive efficiency
Morrell, Jane M. (Swedish University of Agricultural Sciences)
Sabés-Alsina, Maria (Swedish University of Agricultural Sciences)
Abraham, M. C. (Swedish University of Agricultural Sciences)
Sjunnesson, Y. (Swedish University of Agricultural Sciences)

Date:	2016
Abstract:	Several selection techniques are available for processing spermatozoa. Apart from sperm washing to remove seminal plasma, only "swim-up" and colloid centrifugation have been used to any extent to prepare spermatozoa for in vitro fertilization, and only colloid centrifugation has been used to prepare sperm samples for artificial insemination. Single-layer centrifugation (SLC) through a species-specific colloid has been shown to be effective in selecting spermatozoa with good motility, normal morphology and intact chromatin in a range of species. This method is less timeconsuming than swim-up, and has been scaled-up to allow whole ejaculates to be processed in a practical manner. The applications of SLC are as follows: to improve sperm quality in insemination doses or in samples for in vitro fertilization, to increase the shelf life of normal sperm doses, to remove pathogens (viruses, bacteria), to improve cryosurvival by removing dead and dying spermatozoa before freezing or after thawing, to select spermatozoa for intracytoplasmic sperm injection, and to aid conservation breeding.
Grants:	Ministerio de Economía y Competitividad AGL2013-46769-P
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Inseminació artificial ; Fecundació in vitro
Published in:	Animal Reproduction, Vol. 13 Núm. 3 (2016) , p. 340-345

DOI: 10.21451/1984-3143-AR876
PMID: 20814435

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