Four bottlenecks restrict colonization and invasion by the pathogen Ralstonia solanacearum in resistant tomato
Planas-Marquès, Marc (Centre de Recerca en Agrigenòmica)
Kressin, Jonathan P. (North Carolina State University. Department of Entomology and Plant Pathology)
Kashyap, Anurag (Centre de Recerca en Agrigenòmica)
Panthee, Dilip R. (North Carolina State University. Department of Horticultural Science)
Louws, Frank J. (North Carolina State University. Department of Entomology and Plant Pathology)
Sánchez Coll, Núria (Centre de Recerca en Agrigenòmica)
Valls, Marc (Centre de Recerca en Agrigenòmica)

Date:	2020
Abstract:	Ralstonia solanacearum is a bacterial vascular pathogen causing devastating bacterial wilt. In the field, resistance against this pathogen is quantitative and is available for breeders only in tomato and eggplant. To understand the basis of resistance to R. solanacearum in tomato, we investigated the spatio-temporal dynamics of bacterial colonization using non-invasive live monitoring techniques coupled to grafting of susceptible and resistant varieties. We found four 'bottlenecks' that limit the bacterium in resistant tomato: root colonization, vertical movement from roots to shoots, circular vascular bundle invasion, and radial apoplastic spread in the cortex. Radial invasion of cortical extracellular spaces occurred mostly at late disease stages but was observed throughout plant infection. This study shows that resistance is expressed in both root and shoot tissues, and highlights the importance of structural constraints to bacterial spread as a resistance mechanism. It also shows that R. solanacearum is not only a vascular pathogen but spreads out of the xylem, occupying the plant apoplast niche. Our work will help elucidate the complex genetic determinants of resistance, setting the foundations to decipher the molecular mechanisms that limit pathogen colonization, which may provide new precision tools to fight bacterial wilt in the field.
Grants:	Agencia Estatal de Investigación AGL2016-78002-R Ministerio de Economía y Competitividad SEV-2015-0533 Ministerio de Economía y Competitividad RyC2014-16158
Note:	Aquest article té una correcció a 10.1093/jxb/erac086
Note:	Altres ajuts: Generalitat de Catalunya/CERCA Programme USDA/NIFA/2011-51181-30963 USDA/NIFA/2016-51181-25404
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Bacterial wilt ; Disease resistance ; Ralstonia solanacearum ; Tomato ; Vascular pathogen ; Xylem
Published in:	Journal of experimental botany, Vol. 71, Issue 6 (March 2020) , p. 2157-2171, ISSN 1460-2431

Correcció de l'article: https://ddd.uab.cat/record/273902
DOI: 10.1093/jxb/erz562
PMID: 32211785

15 p, 4.6 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles