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CmVPS41 Is a General Gatekeeper for Resistance to Cucumber Mosaic Virus Phloem Entry in Melon
Pascual, Laura (Centre de Recerca en Agrigenòmica)
Jinqiang, Yan (Centre de Recerca en Agrigenòmica)
Pujol, Marta (Centre de Recerca en Agrigenòmica)
Monforte, Antonio J. (Universitat Politècnica de València. Instituto de Biología Molecular y Celular de Plantas)
Picó Silvent, Belén (Instituto de Conservación y Mejora de la Agrodiversidad Valenciana)
Martín-Hernández, Ana Montserrat (Centre de Recerca en Agrigenòmica)

Date: 2019
Abstract: Melon production is often compromised by viral diseases, which cannot be treated with chemicals. Therefore, the use of genetic resistances is the main strategy for generating crops resistant to viruses. Resistance to Cucumber mosaic virus (CMV) in melon is scarcely described in few accessions. Until recently, the only known resistant accessions were Freeman's Cucumber and PI 161375, cultivar Songwhan Charmi (SC). Resistance to CMV in melon is recessive and generally oligogenic and quantitative. However, in SC, the resistance to CMV strains of subgroup II is monogenic, depending only on one gene, cmv1, which is able to stop CMV movement by restricting the virus to the bundle sheath cells and preventing a systemic infection. This restriction depends on the viral movement protein (MP). Chimeric viruses carrying the MP of subgroup II strains, like the strain LS (CMV-LS), are restricted in the bundle sheath cells, whereas those carrying MP from subgroup I, like the strain FNY (CMV-FNY), are able to overcome this restriction. cmv1 encodes a vacuolar protein sorting 41 (CmVPS41), a protein involved in the transport of cargo proteins from the Golgi to the vacuole through late endosomes. We have analyzed the variability of the gene CmVPS41 in a set of 52 melon accessions belonging to 15 melon groups, both from the spp melo and the spp agrestis. We have identified 16 different haplotypes, encoding 12 different CmVPS41 protein variants. Challenging members of all haplotypes with CMV-LS, we have identified nine new resistant accessions. The resistance correlates with the presence of two mutations, either L348R, previously found in the accession SC and present in other three melon genotypes, or G85E, present in Freeman's Cucumber and found also in four additional melon genotypes. Moreover, the new resistant accessions belong to three different melon horticultural groups, Conomon, Makuwa, and Dudaim. In the new resistant accessions, the virus was able to replicate and move cell to cell, but was not able to reach the phloem. Therefore, resistance to phloem entry seems to be a general strategy in melon controlled by CmVPS41. Finally, the newly reported resistant accessions broaden the possibilities for the use of genetic resistances in new melon breeding strategies.
Note: Altres ajuts: Generalitat Valenciana (PROMETEO-2017-078)
Note: Número d'acord de subvenció MINECO/AGL2012-40130-C02-01
Note: Número d'acord de subvenció MINECO/AGL2015-64625-C2-1-R
Note: Número d'acord de subvenció MINECO/SEV-2015-0533
Note: Número d'acord de subvenció MINECO/AGL2015-64625-C2-2-R
Note: Número d'acord de subvenció MCIU/AGL2017-85563-C2-1-R
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès.
Document: article ; recerca ; publishedVersion
Published in: Frontiers in plant science, Vol. 10 (October 2019) , art. 1219, ISSN 1664-462X

DOI: 10.3389/fpls.2019.01219
PMID: 31632432

11 p, 2.5 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles

 Record created 2020-04-09, last modified 2020-06-11

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