Multi-approach analysis to assess the chromium(III) immobilization by Ochrobactrum anthropi DE2010
Villagrasa, Eduard (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Ballesteros, Belén (Institut Català de Nanociència i Nanotecnologia)
Obiol i Plana, Aleix (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Millach, Laia (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Esteve, Isabel (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Solé Cornellà, Antoni (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Date:	2020
Abstract:	Ochrobactrum anthropi DE2010 is a microorganism isolated from Ebro Delta microbial mats and able to resist high doses of chromium(III) due to its capacity to tolerate, absorb and accumulate this metal. The effect of this pollutant on O. anthropi DE2010 has been studied assessing changes in viability and biomass, sorption yields and removal efficiencies. Furthermore, and for the first time, its capacity for immobilizing Cr(III) from culture media was tested by a combination of High Angle Annular Dark Field (HAADF) Scanning Transmission Electron Microscopy (STEM) imaging coupled to Energy Dispersive X-ray spectroscopy (EDX). The results showed that O. anthropi DE2010 was grown optimally at 0-2 mM Cr(III). On the other hand, from 2 to 10 mM Cr(III) microbial plate counts, growth rates, cell viability, and biomass decreased while extracellular polymeric substances (EPS) production increases. Furthermore, this bacterium had a great ability to remove Cr(III) at 10 mM (q = 950. 00 mg g) immobilizing it mostly in bright polyphosphate inclusions and secondarily on the cellular surface at the EPS level. Based on these results, O. anthropi DE2010 could be considered as a potential agent for bioremediation in Cr(III) contaminated environments.
Grants:	Ministerio de Economía y Competitividad CTQ2014-54553-C3-2-R Ministerio de Ciencia e Innovación CGL2008-01891 Ministerio de Economía y Competitividad SEV-2017-0706
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	Ochrobactrum anthropi DE2010 ; CLSM ; ICP-OES ; HAADF-STEM EDX ; Polyphosphate inclusions
Published in:	Chemosphere, Vol. 238 (January 2020) , art. 124663, ISSN 1879-1298

DOI: 10.1016/j.chemosphere.2019.124663
PMID: 31472343

Postprint 38 p, 446.4 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles