Osa-miR7695 enhances transcriptional priming in defense responses against the rice blast fungus
Sánchez Sanuy, Ferran (Centre de Recerca en Agrigenòmica)
Peris Peris, Cristina (Centre de Recerca en Agrigenòmica)
Tomiyama, Shiho (University of Tokyo. Biotechnology Research Center (Japan))
Okada, Kazunori (University of Tokyo. Biotechnology Research Center (Japan))
Hsing, Yue-Ie (Academia Sinica (Taiwan). Institute of Plant and Microbial Biology)
San Segundo, Blanca (Centre de Recerca en Agrigenòmica)
Campo, Sonia (Centre de Recerca en Agrigenòmica)

Date:	2019
Abstract:	Background: MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at the post-transcriptional level in eukaryotes. In rice, MIR7695 expression is regulated by infection with the rice blast fungus Magnaporthe oryzae with subsequent down-regulation of an alternatively spliced transcript of natural resistance-associated macrophage protein 6 (OsNramp6). NRAMP6 functions as an iron transporter in rice. Results: Rice plants grown under high iron supply showed blast resistance, which supports that iron is a factor in controlling blast resistance. During pathogen infection, iron accumulated in the vicinity of M. oryzae appressoria, the sites of pathogen entry, and in cells surrounding infected regions of the rice leaf. Activation-tagged MIR7695 rice plants (MIR7695-Ac) exhibited enhanced iron accumulation and resistance to M. oryzae infection. RNA-seq analysis revealed that blast resistance in MIR7695-Ac plants was associated with strong induction of defense-related genes, including pathogenesis-related and diterpenoid biosynthetic genes. Levels of phytoalexins during pathogen infection were higher in MIR7695-Ac than wild-type plants. Early phytoalexin biosynthetic genes, OsCPS2 and OsCPS4, were also highly upregulated in wild-type rice plants grown under high iron supply. Conclusions: Our data support a positive role of miR7695 in regulating rice immunity that further underpin links between defense and iron signaling in rice. These findings provides a basis to better understand regulatory mechanisms involved in rice immunity in which miR7695 participates which has a great potential for the development of strategies to improve blast resistance in rice.
Grants:	Ministerio de Economía y Competitividad BIO2015-67212-R Ministerio de Economía y Competitividad SEV-2015-0533
Note:	Altres ajuts: CERCA Programme/Generalitat de Catalunya
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Blast ; Defense ; Iron ; Magnaporthe oryzae ; Microrna ; Mir7695 ; Oryza sativa ; Phytoalexins ; Rice ; Transcriptomics
Published in:	BMC plant biology, Vol. 19 (December 2019) , art. 563, ISSN 1471-2229

DOI: 10.1186/s12870-019-2156-5
PMID: 31852430

16 p, 2.0 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
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