Ecologically relevant biomarkers reveal that chronic effects of nitrate depend on sex and life stage in the invasive fish Gambusia holbrooki
Cano-Rocabayera, O. (Universitat de Barcelona. Departament de Biologia Evolutiva)
de Sostoa, Adolf (Universitat de Barcelona. Departament de Biologia Evolutiva)
Padrós, Francesc 1965- (Universitat Autònoma de Barcelona. Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia)
Cárdenas, L. (Universitat de Barcelona. Departament de Biologia Evolutiva)
Maceda-Veiga, A. (Universitat de Barcelona. Departament de Biologia Evolutiva)

Date:	2019
Abstract:	Agricultural intensification and shifts in precipitation regimes due to global climate change are expected to increase nutrient concentrations in aquatic ecosystems. However, the direct effects of nutrients widely present in wastewaters, such as nitrate, are poorly studied. Here, we use multiple indicators of fish health to experimentally test the effects of three ecologically relevant nitrate concentrations (<10, 50 and 250 mg NO /l) on wild-collected mosquitofish (Gambusia holbrooki), a species widely introduced for mosquito biocontrol in often eutrophic waters. Overall, biomarkers (histopathology, feeding assays, growth and caloric content and stable isotopes as indicators of energy content) did not detect overt signs of serious disease in juveniles, males or females of mosquitofish. However, males reduced food intake at the highest nitrate concentration compared to the controls and females. Similarly, juveniles reduced energy reserves without significant changes in growth or food intake. Calorimetry was positively associated with the number of perivisceral fat cells in juveniles, and the growth rate of females was negatively associated with δ N signature in muscle. This study shows that females are more tolerant to nitrate than males and juveniles and illustrates the advantages of combing short- and long-term biomarkers in environmental risk assessment, including when testing for the adequacy of legal thresholds for pollutants.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Peixos ; Animals ; Biomarkers ; Cyprinodontiformes ; Ecosystem ; Environmental Exposure ; Feeding Behavior ; Female ; Male ; Nitrates ; Sex Factors ; Water Pollutants, Chemical
Published in:	PloS one, Vol. 14 Núm. 1 (january 2019) , p. e0211389, ISSN 1932-6203

DOI: 10.1371/journal.pone.0211389
PMID: 30689670

18 p, 1.4 MB

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