Biomarkers of oxidative stress in saliva in pigs : Analytical validation and changes in lactation
Rubio, C. P. (University of Murcia. Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU)
Mainau, Eva (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Cerón, José Joaquín (University of Murcia. Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU)
Contreras-Aguilar, M. D. (University of Murcia. Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU)
Martínez-Subiela, Silvia (University of Murcia. Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU)
Navarro Vives, Elena (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Tecles, Fernando (University of Murcia. Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU)
Manteca Vilanova, Xavier (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Escribano Tortosa, Damián (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)

Date:	2019
Abstract:	Background: Biomarkers of oxidative stress in pigs have been measured in serum/plasma samples. However, blood collection in pigs can be highly stressful to the animals. Saliva is a biological fluid with several advantages in pigs over blood, since it can be easily collected without stress to the animals, being therefore an ideal sample in this species. The objective of this study was the validation of assays for the evaluation of oxidative stress status in saliva of pigs. For this purpose, three assays commonly used to evaluate the total antioxidant capacity (TAC): trolox equivalent antioxidant capacity (TEAC), cupric reducing antioxidant capacity (CUPRAC), and ferric reducing ability of plasma (FRAP)), one individual antioxidant (uric acid) and two assays to evaluate oxidant concentrations (advanced oxidation protein products (AOPP) and hydrogen peroxide (HO)) were measured and validated in porcine saliva. In addition, the possible changes of these assays in sows' saliva during lactation were be studied. Results: The methods had intra- and inter-assays coefficient of variation lower than 15%. They also showed an adequate linearity and recovery, and their detection limits were low enough to detect the analytes in saliva of pigs. Overall the analytical validation tests showed that the assays used in our study are valid and reliable for the evaluation of oxidative stress in porcine saliva. In addition, it was observed that these salivary biomarkers can change in a situation of oxidative stress such as lactation in sows. Conclusions: All assays for salivary biomarkers of oxidative stress evaluated in this study have demonstrated a high analytical accuracy and low imprecision. In addition, it has been observed that these biomarkers showed significant changes in a situation of oxidative stress such as lactation in sows. Therefore, this study opens a new possibility of using saliva as a non-invasive sample to evaluate oxidative stress in pigs.
Note:	This work was supported by the National Council for Scientific and Technological Development (CNPq) of Brazil [200568/2014-0]; the Seneca Foundation of Murcia Regional Government, Spain [grant number 19894/ GERM/15]; the 'Juan de la Cierva' of the 'Ministerio de Economía y Competitividad', Spain.; and the University of Murcia (R-605/2016), Spain. The funding agencies had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Note:	Altres ajuts: CNPq/200568/2014-0
Note:	Altres ajuts: UM/R-605/2016
Note:	Altres ajuts: Seneca/19894/GERM/15
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Porcs ; Efectes de l'estrès sobre els animals ; Sows ; Antioxidant ; Oxidant ; Reactive oxygen species
Published in:	BMC veterinary research, Vol. 15 Núm. 1 (14 2019) , p. 144, ISSN 1746-6148

DOI: 10.1186/s12917-019-1875-z
PMID: 31088564

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