Biofunctionalization with a TGFβ-1 inhibitor peptide in the osseointegration of synthetic bone grafts : An in vivo study in beagle dogs
Cirera, Andrea (Oral Surgery and Implant Dentistry Department. School of Dentistry. University of Granada)
Manzanares, María-Cristina (Universitat de Barcelona. Departament de Patologia i Terapèutica Experimental)
Sevilla, P. (Escola Universitària Salesiana de Sarrià)
Ortiz-Hernandez, Monica (Biomaterials. Biomechanics and)
Galindo-Moreno, P. (Universidad de Granada. Oral Surgery and Implant Dentistry Department.)
Gil Mur, Javier (Universitat Internacional de Catalunya. Bioengineering Institute of Technology)

Date:	2019
Abstract:	Objectives: The aim of this research was to determine the osseointegration of two presentations of biphasic calcium phosphate (BCP) biomaterial-one untreated and another submitted to biofunctionalization with a TGF-β1 inhibitor peptide, P144, on dental alveolus. Materials and Methods: A synthetic bone graft was used, namely, (i) Maxresorb® (Botiss Klockner) (n = 12), and (ii) Maxresorb® (Botiss Klockner) biofunctionalized with P144 peptide (n = 12). Both bone grafts were implanted in the two hemimandibles of six beagle dogs in the same surgical time, immediately after tooth extraction. Two dogs were sacrificed 2, 4, and 8 weeks post implant insertion, respectively. The samples were submitted to histomorphometrical and histological analyses. For each sample, we quantified the new bone growth and the new bone formed around the biomaterial's granules. After optical microscopic histological evaluation, selected samples were studied using backscattered scanning electron microscopy (BS-SEM). Results: The biofunctionalization of the biomaterial's granules maintains a stable membranous bone formation throughout the experiment timeline, benefitting from the constant presence of vascular structures in the alveolar space, in a more active manner that in the control samples. Better results in the experimental groups were proven both by quantitative and qualitative analysis. Conclusions: Synthetic bone graft biofunctionalization results in slightly better quantitative parameters of the implant's osseointegration. The qualitative histological and ultramicroscopic analysis shows that biofunctionalization may shorten the healing period of dental biomaterials.
Note:	Altres ajuts: FEDER/RTI2018-098075-B-C22
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Osseointegration ; Biofunctionalization ; Histomorphometrical analysis ; Scanning electron microscopy ; Synthetic bone graft ; TGF-β1
Published in:	Materials, Vol. 12 Núm. 19 (january 2019) , p. 3168, ISSN 1996-1944

DOI: 10.3390/ma12193168
PMID: 31569702

23 p, 5.8 MB

The record appears in these collections:
Articles > Research articles
Articles > Published articles