Toxicogenomics of gold nanoparticles in a marine fish : Linkage to classical biomarkers
Teles, Mariana (CIIMAR-Interdisciplinary Centre of Marine and Environmental Research. Terminal de Cruzeiros do Porto de Leixões)
Reyes-López, F. E. (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Balasch Alemany, Joan Carles (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Tvarijonaviciute, A. (Interdisciplinary Laboratory of Clinical Analysis INTERLAB-UMU. Regional Campus of International Excellence Mare Nostrum. University of Murcia)
Guimarães, L. (CIIMAR-Interdisciplinary Centre of Marine and Environmental Research. Terminal de Cruzeiros do Porto de Leixões)
Oliveira, M. (Department of Biology and CESAM, University of Aveiro)
Tort, L. (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)

Data:	2019
Resum:	In the present study, the underlying short-term effects of gold nanoparticles (AuNP, spheres, citrate coated, -40 nm) on the hepatic function of gilthead sea bream (Sparus aurata) was assessed, using a species-specific enriched oligonucleotide microarray platform (SAQ). Two distinct concentrations of AuNP (0. 5 and 50 μg/L) were tested during 24 h waterborne exposure. The transcriptional profile was complemented with outcomes at higher levels of biological organization, including hepatic health indicators and DNA damage indicators. DNA damaging potential of AuNP was assessed in whole peripheral blood, assessing DNA strand breaks (using the comet assay) and chromosome damage (scoring the erythrocytic nuclear abnormalities, ENA). The overall genetic response showed a differential hepatic transcriptional profile, both in terms of number and intensity, of differentially expressed genes (DEG). Concerning the functional pathways that were affected, the main changes found were for gene encoding proteins involved in the response to xenobiotics, oxidoreductase activity, immunomodulation, DNA repair and programmed cell death types I and II. The hepatic health indicators measured revealed that AuNP can induce liver injury, as demonstrated by the plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) significantly increased activities after exposure to the highest AuNP concentration (50 μg/L). Exposure to AuNP also caused DNA strand breaks, however, without causing clastogenesis or aneugenesis, since no ENA were detected. Overall, data showed that a short-term exposure to AuNP can modulate gene expression in liver and affects several biochemical/genetic functions in fish.
Nota:	Càrrega feta de Scopus d'articles UAB 2019 (Gold, hybrid o Bronze) procedents de l'Observatori d'Accés Obert (càrrega maig 2020). Compte! Cal comprovar la versió permesa per l'editor en els bronze.
Drets:	Tots els drets reservats.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	Frontiers in marine science, Vol. 6 Núm. APR (2019) , p. 147, ISSN 2296-7745

DOI: 10.3389/fmars.2019.00147

12 p, 4.8 MB

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