Addition of chlorogenic acid and caffeine during the processing of cooled boar semen
Pereira, B. A. (Universidade Federal de Lavras)
Rocha, L. G. P. (Universidade Federal de Lavras)
Teles, M. C. (Universidade Federal de Lavras)
Silva, W. E. (Universidade Federal de Lavras)
Barbosa, J. A. (Universidade Federal de Lavras)
Rabelo, S. S. (Universidade Federal de Lavras)
Uchoa, A. S. (Universidade Federal de Lavras)
Rodríguez Gil, Joan Enric. (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Pereira, L. J. (Universidade Federal de Lavras)
Zangeronimo, Marcio Gilberto (Universidade Federal de Lavras)

Data:	2019
Resum:	A study was conducted to evaluate the effect of chlorogenic acid (ChA) added pre-cooling and its combination with caffeine added during warming on cooled-stored boar semen parameters. Ten ejaculates were diluted in commercial extender with or without 4. 5mg/ml ChA and stored at 15°C. After 0, 24 and 72 hours of storage, aliquots of these doses were taken and incubated at 37°C in the presence or absence of 8. 0mM caffeine. Semen quality was evaluated after 10 and 120 minutes of incubation. The ChA increased (P < 0. 01) the sperm motility, viability, acrosomal integrity and the percentage of spermatozoa with high mitochondrial activity (PMHA), however, decreased (P < 0. 01) the malondialdehyde (MDA) concentration. Caffeine increased (P < 0. 05) the sperm motility, viability, PMHA and the MDA concentration and reduced (P < 0. 05) the acrosome integrity. When associated (ChA+caffeine), there was an increase (P < 0. 05) in sperm motility and viability, PMHA and acrosome integrity. The addition of ChA to the dilution medium improves the quality of the swine inseminating doses. The addition of caffeine during re-warming is only recommended when the semen is stored for prolonged periods (72h), and the inseminating dose should be used immediately after its addition.
Nota:	The authors thank CAPES (PNPD Institucional, process number 2457/2011, and Programa Pesquisador Visitante Especial - PVE's, process number 88881.030399/2013-01), FAPEMIG (PPM-00460-12), CNPq (305478/2015-0 and 446288/2014-4), Minitub do Brasil, Fazenda São Paulo and the Postgraduate Program in Veterinary Sciences UFLA for their support.
Nota:	Altres ajuts: CNPq/305478/2015-0
Nota:	Altres ajuts: CNPq/446288/2014-4
Nota:	Altres ajuts: CAPES/2457/2011
Nota:	Altres ajuts: CAPES/88881.030399/2013-01
Nota:	Altres ajuts: FAPEMIG/PPM-00460-12
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Boar semen storage ; Metabolic activator ; Methylxanthine ; Polyphenol ; Antioxidant
Publicat a:	Arquivo brasileiro de medicina veterinária e zootecnia, Vol. 71 Núm. 2 (january 2019) , p. 489-499, ISSN 1678-4162

DOI: 10.1590/1678-4162-10415

11 p, 737.8 KB

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