The biological potential hidden in inclusion bodies
Gifre-Renom, Laia (Institut de Recerca i Tecnologia Agroalimentàries)
Seras-Franzoso, Joaquin (Hospital Universitari Vall d'Hebron)
Rafael, Diana (Hospital Universitari Vall d'Hebron)
Andrade, Fernanda (Hospital Universitari Vall d'Hebron)
Cano-Garrido, Olivia (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Martinez-Trucharte, Francesc (Hospital Universitari Vall d'Hebron)
Ugarte-Berzal, Estefanía (Katholieke Universiteit Leuven. Department of Microbiology, Immunology and Transplantation)
Martens, Erik (Katholieke Universiteit Leuven. Department of Microbiology, Immunology and Transplantation)
Boon, Lise (Katholieke Universiteit Leuven. Department of Microbiology, Immunology and Transplantation)
Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Opdenakker, Ghislain (Katholieke Universiteit Leuven. Department of Microbiology, Immunology and Transplantation)
Schwartz, Simon (Hospital Universitari Vall d'Hebron)
Arís i Giralt, Anna (Institut de Recerca i Tecnologia Agroalimentàries)
Garcia-Fruitos, Elena (Institut de Recerca i Tecnologia Agroalimentàries)

Fecha:	2020
Resumen:	Inclusion bodies (IBs) are protein nanoclusters obtained during recombinant protein production processes, and several studies have demonstrated their potential as biomaterials for therapeutic protein delivery. Nevertheless, IBs have been, so far, exclusively sifted by their biological activity in vitro to be considered in further protein-based treatments in vivo. Matrix metalloproteinase-9 (MMP-9) protein, which has an important role facilitating the migration of immune cells, was used as model protein. The MMP-9 IBs were compared with their soluble counterpart and with MMP-9 encapsulated in polymeric-based micelles (PM) through ionic and covalent binding. The soluble MMP-9 and the MMP-9-ionic PM showed the highest activity values in vitro. IBs showed the lowest activity values in vitro, but the specific activity evolution in 50% bovine serum at room temperature proved that they were the most stable format. The data obtained with the use of an air-pouch mouse model showed that MMP-9 IBs presented the highest in vivo activity compared to the soluble MMP-9, which was associated only to a low and a transitory peak of activity. These results demonstrated that the in vivo performance is the addition of many parameters that did not always correlate with the in vitro behavior of the protein of interest, becoming especially relevant at evaluating the potential of IBs as a protein-based nanomaterial for therapeutic purposes.
Ayudas:	Ministerio de Economía y Competitividad RTA2015-00064-C02 Ministerio de Economía y Competitividad RTA2012-00028-C02 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-229
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Inclusion body ; Polymeric micelle ; Stability ; Matrix metalloproteinase-9 ; In vitro ; In vivo
Publicado en:	Pharmaceutics, Vol. 12, issue 2 (2020) , art. 157, ISSN 1999-4923

DOI: 10.3390/pharmaceutics12020157
PMID: 32075316

12 p, 2.9 MB

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Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Instituto de Biotecnología y de Biomedicina (IBB)
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