Glycosylated amyloid-like proteins in the structural extracellular polymers of aerobic granular sludge enriched with ammonium-oxidizing bacteria
Lin, Yuemei (Delft University of Technology. Department of Biotechnology)
Reino, Clara (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Carrera, Julian (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Pérez Cañestro, Julio (Delft University of Technology. Department of Biotechnology)
van Loosdrecht, Mark C. M. (Delft University of Technology. Department of Biotechnology)
Date: |
2018 |
Abstract: |
A new type of structural extracellular polymers () was extracted from aerobic granular sludge dominated by ammonium-oxidizing bacteria. It was analyzed by Raman and spectroscopy to characterize specific amino acids and protein secondary structure, and by - with different stains to identify different glycoconjugates. Its intrinsic fluorescence was captured to visualize the location of the extracted in the nitrifying granules, and its hydrogel-forming property was studied by rheometry. The extracted is abundant with cross ß-sheet secondary structure, contains glycosylated proteins/polypeptides, and rich in tryptophan. It forms hydrogel with high mechanical strength. The extraction and discovery of glycosylated proteins and/or amyloids further shows that conventionally used extraction and characterization techniques are not adequate for the study of structural extracellular polymers in biofilms and/or granular sludge. Confirming amyloids secondary structure in such a complex sample is challengeable due to the possibility of amyloids glycosylation and self-assembly. A new definition of extracellular polymers components which includes glycosylated proteins and a better approach to studying them is required to stimulate biofilm research. |
Grants: |
European Commission 326705
|
Rights: |
Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
Language: |
Anglès |
Document: |
Article ; recerca ; Versió publicada |
Subject: |
Aerobic granular sludge ;
Ammonium-oxidizing bacteria ;
Amyloid ;
Extracellular polymeric substances ;
Glycoproteins |
Published in: |
MicrobiologyOpen, Vol. 7, issue 6 (Dec. 2018) , ISSN 2045-8827 |
DOI: 10.1002/mbo3.616
PMID: 29604180
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Record created 2020-07-13, last modified 2022-02-06