Glycosylated amyloid-like proteins in the structural extracellular polymers of aerobic granular sludge enriched with ammonium-oxidizing bacteria
Lin, Yuemei (Delft University of Technology. Department of Biotechnology)
Reino, Clara (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Carrera, Julian (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Pérez Cañestro, Julio (Delft University of Technology. Department of Biotechnology)
van Loosdrecht, Mark C. M. (Delft University of Technology. Department of Biotechnology)

Data:	2018
Resum:	A new type of structural extracellular polymers () was extracted from aerobic granular sludge dominated by ammonium-oxidizing bacteria. It was analyzed by Raman and spectroscopy to characterize specific amino acids and protein secondary structure, and by - with different stains to identify different glycoconjugates. Its intrinsic fluorescence was captured to visualize the location of the extracted in the nitrifying granules, and its hydrogel-forming property was studied by rheometry. The extracted is abundant with cross ß-sheet secondary structure, contains glycosylated proteins/polypeptides, and rich in tryptophan. It forms hydrogel with high mechanical strength. The extraction and discovery of glycosylated proteins and/or amyloids further shows that conventionally used extraction and characterization techniques are not adequate for the study of structural extracellular polymers in biofilms and/or granular sludge. Confirming amyloids secondary structure in such a complex sample is challengeable due to the possibility of amyloids glycosylation and self-assembly. A new definition of extracellular polymers components which includes glycosylated proteins and a better approach to studying them is required to stimulate biofilm research.
Ajuts:	European Commission 326705
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Aerobic granular sludge ; Ammonium-oxidizing bacteria ; Amyloid ; Extracellular polymeric substances ; Glycoproteins
Publicat a:	MicrobiologyOpen, Vol. 7, issue 6 (Dec. 2018) , ISSN 2045-8827

DOI: 10.1002/mbo3.616
PMID: 29604180

13 p, 898.1 KB

El registre apareix a les col·leccions:
Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Enginyeries > GENOCOV
Articles > Articles de recerca
Articles > Articles publicats