Protease activities triggered by Ralstonia solanacearum infection in susceptible and tolerant tomato lines
Planas-Marquès, Marc (Centre de Recerca en Agrigenòmica)
Bernardo-Faura, Martí (Centre de Recerca en Agrigenòmica)
Paulus, Judith Katharina (University of Oxford. Department of Plant Sciences. Plant Chemetics Laboratory (UK))
Kaschani, Farnusch (Universität Duisburg-Essen. Chemische Biologie, Zentrum für Medizinische Biotechnologie (Germany))
Kaiser, Markus (Universität Duisburg-Essen. Chemische Biologie, Zentrum für Medizinische Biotechnologie (Germany))
Valls, Marc (Centre de Recerca en Agrigenòmica)
van der Hoorn, Renier (University of Oxford. Department of Plant Sciences. Plant Chemetics Laboratory (UK))
Sánchez Coll, Núria (Centre de Recerca en Agrigenòmica)

Date:	2018
Abstract:	Activity-based protein profiling (ABPP) is a powerful proteomic technique to display protein activities in a proteome. It is based on the use of small molecular probes that react with the active site of proteins in an activity-dependent manner. We used ABPP to dissect the protein activity changes that occur in the intercellular spaces of tolerant (Hawaii 7996) and susceptible (Marmande) tomato plants in response to R. solanacearum, the causing agent of bacterial wilt, one of the most destructive bacterial diseases in plants. The intercellular space -or apoplast- is the first battlefield where the plant faces R. solanacearum. Here, we explore the possibility that the limited R. solanacearum colonization reported in the apoplast of tolerant tomato is partly determined by its active proteome. Our work reveals specific activation of papain-like cysteine proteases (PLCPs) and serine hydrolases (SHs) in the leaf apoplast of the tolerant tomato Hawaii 7996 on R. solanacearum infection. The P69 family members P69C and P69F, and an unannotated lipase (Solyc02g077110. 2. 1), were found to be post-translationally activated. In addition, protein network analysis showed that deeper changes in network topology take place in the susceptible tomato variety, suggesting that the tolerant cultivar might be more prepared to face R. solanacearum in its basal state. Altogether this work identifies significant changes in the activity of 4 PLCPs and 27 SHs in the tomato leaf apoplast in response to R. solanacearum, most of which are yet to be characterized. Our findings denote the importance of novel proteomic approaches such as ABPP to provide new insights on old and elusive questions regarding the molecular basis of resistance to R. solanacearum.
Grants:	Ministerio de Economía y Competitividad AGL2013-46898-R Ministerio de Economía y Competitividad RyC-2014-16158 European Commission 331392 European Commission 258413 European Commission 616449 Ministerio de Economía y Competitividad SEV2015-0533
Note:	Altres ajuts: CERCA Programme/Generalitat de Catalunya
Rights:	Tots els drets reservats.
Language:	Anglès
Document:	Article ; recerca ; Versió sotmesa a revisió
Subject:	Activity-based protein profiling ; Bacterial wilt ; Disease resistance ; Papain-like cysteine protease ; Proteomics ; Ralstonia solanacearum ; Serine hydrolase ; Tomato
Published in:	Molecular & Cellular Proteomics, Vol. 17, Issue 6 (March 2018) , p. 1112-1125, ISSN 1535-9476

DOI: 10.1074/mcp.RA117.000052
PMID: 29523767

Preprint 49 p, 1.2 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles