Cardiovascular Risk Factors and Differential Transcriptomic Profile of the Subcutaneous and Visceral Adipose Tissue and Their Resident Stem Cells
Arderiu, Gemma (Institut d'Investigació Biomèdica Sant Pau)
Ballesta, Carlos (Centro Médico Teknon. Barcelona)
Moscatiello, Fabrizio (Centro Médico Teknon. Barcelona)
Vilahur, Gemma (Institut d'Investigació Biomèdica Sant Pau)
Badimon, Lina (Institut d'Investigació Biomèdica Sant Pau)
Lambert, Carmen (Institut d'Investigació Biomèdica Sant Pau)
Universitat Autònoma de Barcelona

Fecha:	2020
Resumen:	Background: The increase in the incidence of obesity and obesity-related cardiovascular risk factors (CVRFs) over the last decades has brought attention on adipose tissue (AT) pathobiology. The expansion of AT is associated with the development of new vasculature needed to perfuse the tissue; however, not all fat depots have the same ability to induce angiogenesis that requires recruitment of their own endothelial cells. In this study we have investigated the effect of different CVRFs, on the angiogenic capacity of the subcutaneous (SAT) and visceral (VAT) adipose tissue and on the function of their mesenchymal cell reservoir. Methods: A transcriptomic approach was used to compare the different angiogenic and inflammatory profiles of the subcutaneous and visceral fat depots from individuals with obesity, as well as their resident stem cells (ASCs). Influence of other risk factors on fat composition was also measured. Finally, the microvesicles (MVs) released by ASCs were isolated and their regenerative potential analyzed by molecular and cellular methodologies. Results: Obesity decreases the angiogenic capacity of AT. There are differences between SAT and VAT; from the 21 angiogenic-related genes analyzed, only three were decreased in SAT compared with those decreased in VAT. ASCs isolated from both fat depots showed significant differences; there was a significant up-regulation of the VEGF-pathway on visceral derived ASCs. ASCs release MVs that stimulate endothelial cell migration and angiogenic capacity. Conclusions: In patients with obesity, SAT expresses a greater number of angiogenic molecules than VAT, independent of the presence of other CVRFs.
Ayudas:	Agencia Estatal de Investigación SAF2016-76819-R Ministerio de Economía y Competitividad TERCELRD16/0011/0018 Instituto de Salud Carlos III CB16-11-0041 Instituto de Salud Carlos III P17-01321 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-1480
Nota:	Altres ajuts: This work was supported by grants from FEDER "Una Manera de Hacer Europa"; the Secretary of University and Research. We thank FIC-Fundacion Jesús Serra, Barcelona, Spain, for their continuous support.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Adipose stem cells ; Endothelial cells ; Microvesicles ; Obesity ; Cardiovascular risk factors ; And angiogenesis
Publicado en:	Cells, Vol. 9 Núm. 10 (2020) , p. 2235, ISSN 2073-4409

DOI: 10.3390/cells9102235
PMID: 33022994

20 p, 5.1 MB

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