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Página principal > Artículos > Artículos publicados > Epigenetic differences in the innate response after immune stimulation during zebrafish sex differentiation |
Fecha: | 2021 |
Resumen: | Infections are able to trigger epigenetic modifications; however, epigenetic-mediating infections in the immune system in fish is currently unavailable. Within this purpose, zebrafish were immune-stimulated with three lipopolysaccharides (LPS) during sex differentiation. Methylation patterns of three immune genes were studied by a candidate gene approach together with gene expression analysis, and in adulthood, sex ratios were determined. It was shown that the entrance of LPS was through the gills and accumulated in the pronephros. Significant hypomethylation levels of CASP9 and a significant CpG site for IL1β after Pseudomonas aeruginosa LPS exposure were found. No methylation difference was observed for TNFα. Gene expression and correlation data differed among studied genes. Sex ratios showed a feminization in dose and LPS strain-dependent manner. Here, it is provided epigenetic regulatory mechanisms derived by innate response and the first evidence of possible epigenetic interactions between the immune and reproductive systems. |
Ayudas: | Ministerio de Ciencia e Innovación AGL2015-73864-JIN Ministerio de Ciencia e Innovación RYC2018-024017-I Ministerio de Ciencia e Innovación 202030E004 Ministerio de Economía y Competitividad RTI2018-096957-B-C21 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-345 |
Derechos: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. |
Lengua: | Anglès |
Documento: | Article ; recerca ; Versió acceptada per publicar |
Materia: | Gonads ; Immune ; LPS ; Methylation ; Reproduction ; Sex ratio ; Zebrafish |
Publicado en: | Developmental and Comparative Immunology, Vol. 114 (January 2021) , art. 103848, ISSN 0145-305X |
Postprint 40 p, 2.2 MB |