Ulcerative Colitis Induces Changes on the Expression of the Endocannabinoid System in the Human Colonic Tissue
Márquez-Mosquera, Lucía (Hospital del Mar (Barcelona, Catalunya))
Suárez, Juan (Fundación Pública Andaluza para la Investigación de Málaga en Biomedicina y Salud)
Iglesias, Mar (Universitat Autònoma de Barcelona. Departament de Ciències Morfològiques)
Bermudez-Silva, Francisco Javier (Fundación Pública Andaluza para la Investigación de Málaga en Biomedicina y Salud)
Rodríguez de Fonseca, Fernando (Fundación Pública Andaluza para la Investigación de Málaga en Biomedicina y Salud)
Andreu, Montserrat (Hospital del Mar (Barcelona, Catalunya))

Data:	2009
Resum:	Recent studies suggest potential roles of the endocannabinoid system in gastrointestinal inflammation. Although cannabinoid CB receptor expression is increased in inflammatory disorders, the presence and function of the remaining proteins of the endocannabinoid system in the colonic tissue is not well characterized. Cannabinoid CB and CB receptors, the enzymes for endocannabinoid biosynthesis DAGLα, DAGLβ and NAPE-PLD, and the endocannabinoid-degradating enzymes FAAH and MAGL were analysed in both acute untreated active ulcerative pancolitis and treated quiescent patients in comparison with healthy human colonic tissue by immunocytochemistry. Analyses were carried out according to clinical criteria, taking into account the severity at onset and treatment received. Western blot and immunocytochemistry indicated that the endocannabinoid system is present in the colonic tissue, but it shows a differential distribution in epithelium, lamina propria, smooth muscle and enteric plexi. Quantification of epithelial immunoreactivity showed an increase of CB receptor, DAGLα and MAGL expression, mainly in mild and moderate pancolitis patients. In contrast, NAPE-PLD expression decreased in moderate and severe pancolitis patients. During quiescent pancolitis, CB, CB and DAGLα expression dropped, while NAPE-PLD expression rose, mainly in patients treated with 5-ASA or 5-ASA+corticosteroids. The number of immune cells containing MAGL and FAAH in the lamina propria increased in acute pancolitis patients, but dropped after treatment. Endocannabinoids signaling pathway, through CB receptor, may reduce colitis-associated inflammation suggesting a potential drugable target for the treatment of inflammatory bowel diseases.
Ajuts:	Instituto de Salud Carlos III FIS07-0880 Instituto de Salud Carlos III FIS07-1226 Instituto de Salud Carlos III RD06-001
Nota:	Altres ajuts: Proyectos de Excelencia, Consejeria de Innovacion de la Junta de Andalucia; Marato TV3 2005/060; Beca Iniciacion a la Recerca 2008. Societat Catalana Digestologia.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Cannabinoid Receptor Modulators ; Colitis, Ulcerative ; Colon ; Endocannabinoids ; Gastrointestinal Tract ; Gene Expression Regulation ; Humans ; Inflammation ; Metabolism ; Physiopathology
Publicat a:	PloS one, Vol. 4 (september 2009) , ISSN 1932-6203

DOI: 10.1371/journal.pone.0006893
PMID: 19730730

13 p, 2.8 MB

El registre apareix a les col·leccions:
Articles > Articles de recerca
Articles > Articles publicats