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Pàgina inicial > Articles > Articles publicats > Nano-lantern on paper for smartphone-based ATP detection |
Data: | 2020 |
Resum: | ATP-driven bioluminescence relying on the D-luciferin-luciferase reaction is widely employed for several biosensing applications where bacterial ATP detection allows to verify microbial contamination for hygiene monitoring in hospitals, food processing and in general for cell viability studies. Several ATP kit assays are already commercially available but an user-friendly ATP biosensor characterized by low-cost, portability, and adequate sensitivity would be highly valuable for rapid and facile on site screening. Thanks to an innovative freeze-drying procedure, we developed a user-friendly, ready-to-use and stable ATP sensing paper biosensor that can be combined with smartphone detection. The ATP sensing paper includes a lyophilized "nano-lantern" with reaction components being rapidly reconstituted by 10 μL sample addition, enabling detection of 10 mol of ATP within 10 min. We analysed urinary microbial ATP as a biomarker of urinary tract infection (UTI), confirming the capability of the ATP sensing paper to detect the threshold for positivity corresponding to 10 colony-forming units of bacteria per mL of urine. |
Ajuts: | Ministerio de Economía y Competitividad SEV-2017-0706 Ministerio de Economía y Competitividad MAT2017-87202-P |
Nota: | Altres ajuts: the ICN2 is funded by the CERCA Programme / Generalitat de Catalunya. |
Drets: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. |
Llengua: | Anglès |
Document: | Article ; recerca ; Versió acceptada per publicar |
Matèria: | Bioluminescence ; ATP biosensor ; Luciferase ; Smartphone ; Paper-based biosensor |
Publicat a: | Biosensors & bioelectronics, Vol. 150 (Feb. 2020) , art. 111902, ISSN 1873-4235 |
Postprint 26 p, 1.2 MB |