Inkjet-printed electrochemically reduced graphene oxide microelectrode as a platform for HT-2 mycotoxin immunoenzymatic biosensing
Kudr, Jiri (Institut Català de Nanociència i Nanotecnologia)
Zhao, Lei (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Nguyen, Emily P. (Institut Català de Nanociència i Nanotecnologia)
Arola, Henri (VTT Technical Research Centre of Finland)
Nevanen, Tarja K. (VTT Technical Research Centre of Finland)
Adam, Vojtech (Brno University of Technology. Central European Institute of Technology)
Zitka, Ondrej (Brno University of Technology. Central European Institute of Technology)
Merkoçi, Arben (Institut Català de Nanociència i Nanotecnologia)

Data:	2020
Resum:	The design and application of an inkjet-printed electrochemically reduced graphene oxide microelectrode for HT-2 mycotoxin immunoenzymatic biosensing is reported. A water-based graphene oxide ink was first formulated and single-drop line working microelectrodes were inkjet-printed onto poly(ethylene 2,6-naphthalate) substrates, with dimensions of 78 μm in width and 30 nm in height after solvent evaporation. The printed graphene oxide microelectrodes were electrochemically reduced and characterized by Raman and X-ray photoelectron spectroscopies in addition to microscopies. Through optimization of the electrochemical reduction parameters, differential pulse voltammetry were performed to examine the sensing of 1-naphthol (1-N), where it was revealed that reduction times had significant effects on electrode performance. The developed microelectrodes were then used as an immunoenzymatic biosensor for the detection of HT-2 mycotoxin based on carbodiimide linking of the microelectrode surface and HT-2 toxin antigen binding fragment of antibody (anti-HT2 (10) Fab). The HT-2 toxin and anti-HT2 (10) Fab reaction was reported by anti-HT2 immune complex single-chain variable fragment of antibody fused with alkaline phosphatase (anti-IC-HT2 scFv-ALP) which is able to produce an electroactive reporter - 1-N. The biosensor showed detection limit of 1. 6 ng ∙ mL¯¹ and a linear dynamic range of 6. 3 - 100. 0 ng ∙ mL¯¹ within a 5 min incubation with 1-naphthyl phosphate (1-NP) substrate.
Ajuts:	European Commission 785219 Agencia Estatal de Investigación MAT2017-87202-P Ministerio de Economía y Competitividad SEV2201320295
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Matèria:	2D material ; Antibody ; Biosensor ; Electrochemistry ; Graphene oxide
Publicat a:	Biosensors & bioelectronics, Vol. 156 (May 2020) , art. 112109, ISSN 1873-4235

DOI: 10.1016/j.bios.2020.112109

Postprint 23 p, 903.7 KB

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