The biomolecule corona of lipid nanoparticles contains circulating cell-free DNA
Gardner, Lois (The University of Manchester. Nanomedicine Lab)
Warrington, Jessica (The University of Manchester. Nanomedicine Lab)
Rogan, Jane (Manchester Cancer Research Centre Biobank)
Rothwell, Dominic G. (University of Manchester. Manchester Institute Cancer Biomarker Centre)
Brady, Ged (University of Manchester. Manchester Institute Cancer Biomarker Centre)
Dive, Caroline (University of Manchester. Manchester Institute Cancer Biomarker Centre)
Kostarelos, Kostas (Institut Català de Nanociència i Nanotecnologia)
Hadjidemetriou, Marilena (University of Manchester. Nanomedicine Lab.)

Date:	2020
Abstract:	The spontaneous adsorption of biomolecules onto the surface of nanoparticles (NPs) in complex physiological biofluids has been widely investigated over the last decade. Characterisation of the protein composition of the 'biomolecule corona' has dominated research efforts, whereas other classes of biomolecules, such as nucleic acids, have received no interest. Scarce, speculative statements exist in the literature about the presence of nucleic acids in the biomolecule corona, with no previous studies attempting to describe the contribution of genomic content to the blood-derived NP corona. Herein, we provide the first experimental evidence of the interaction of circulating cell-free DNA (cfDNA) with lipid-based NPs upon their incubation with human plasma samples, obtained from healthy volunteers and ovarian carcinoma patients. Our results also demonstrate an increased amount of detectable cfDNA in patients with cancer. Proteomic analysis of the same biomolecule coronas revealed the presence of histone proteins, suggesting an indirect, nucleosome-mediated NP-cfDNA interaction. The finding of cfDNA as part of the NP corona, offers a previously unreported new scope regarding the chemical composition of the 'biomolecule corona' and opens up new possibilities for the potential exploitation of the biomolecule corona for the enrichment and analysis of blood-circulating nucleic acids.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Chemical compositions ; Experimental evidence ; Human plasma samples ; Lipid nanoparticles ; Nanoparticle (NPs) ; Protein composition ; Proteomic analysis ; Spontaneous adsorption
Published in:	Nanoscale horizons, Vol. 5, issue 11 (2020) , p. 1476-1486, ISSN 2055-6764

DOI: 10.1039/d0nh00333f

12 p, 4.0 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles