Activation of regulated cell death in the lung of piglets infected with virulent PRRSV-1 Lena strain occurs earlier and mediated by cleaved Caspase-8
Sánchez-Carvajal, Jose María (University of Córdoba. Department of Anatomy and Comparative Pathology and Toxicology)
Ruedas-Torres, Inés (University of Córdoba. Department of Anatomy and Comparative Pathology and Toxicology)
Carrasco, Librado (University of Córdoba. Department of Anatomy and Comparative Pathology and Toxicology)
Pallarés Martínez, Francisco José (University of Córdoba. Department of Anatomy and Comparative Pathology and Toxicology)
Mateu de Antonio, Enrique María (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Rodríguez-Gómez, Irene Magdalena (University of Córdoba. Department of Anatomy and Comparative Pathology and Toxicology, Faculty of Veterinary Medicine)
Gómez-Laguna, Jaime (University of Córdoba. Department of Anatomy and Comparative Pathology and Toxicology)

Fecha:	2021
Resumen:	PRRSV-1 virulent strains cause high fever, marked respiratory disease and severe lesions in lung and lymphoid organs. Regulated cell death (RCD), such as apoptosis, necroptosis and pyroptosis, is triggered by the host to interrupt viral replication eliminating infected cells, however, although it seems to play a central role in the immunopathogenesis of PRRSV, there are significant gaps regarding their sequence and activation upon PRRSV-infection. The present study evaluated RCD events by means of caspases expression in the lung of PRRSV-1-infected pigs and their impact on pulmonary macrophage subpopulations and lung lesion. Conventional piglets were intranasally inoculated with the virulent subtype 3 Lena strain or the low virulent subtype 1 3249 strain and euthanised at 1, 3, 6, 8 and 13 dpi. Lena-infected piglets showed severe and early lung damage with a high frequency of PRRSV-N-protein + cells, depletion of CD163 + cells and high viral load in the lung. The number of TUNEL + cells was significantly higher than cCasp3 + cells in Lena-infected piglets during the first week post-infection. cCasp8 and to a lesser extent cCasp9 were activated by both PRRSV-1 strains after one week post-infection together with a replenishment of both CD163 + and Arg-1 + pulmonary macrophages. These results highlight the induction of other forms of RCD beyond apoptosis, such as, necroptosis and pyroptosis during the first week post-infection followed by the activation of, mainly, extrinsic apoptosis during the second week post-infection. The recovery of CD163 + macrophages at the end of the study represents an attempt to restore pulmonary macrophage subpopulations lost during the early stages of the infection but also a macrophage polarisation into M2 macrophages.
Ayudas:	Ministerio de Economía y Competitividad AGL2016-76111-R Ministerio de Economía y Competitividad RyC-2014-16735 Ministerio de Economía y Competitividad PID2019-109718GB-I00
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	PRRSV-1 ; Virulent strain ; Regulated cell death ; Lung ; Cleaved caspase-8
Publicado en:	Veterinary research, Vol. 52 (january 2021) , ISSN 1297-9716

DOI: 10.1186/s13567-020-00882-x
PMID: 33482914

14 p, 7.6 MB

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Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
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