Resynthesis : marker-based partial reconstruction of elite genotypes in clonally-reproducing plant species
Eduardo, Iban (Centre de Recerca en Agrigenòmica)
Alegre, Simó (Institut de Recerca i Tecnologia Agroalimentàries)
Alexiou, Konstantinos G. (Centre de Recerca en Agrigenòmica)
Arús i Gorina, Pere (Centre de Recerca en Agrigenòmica)

Date:	2020
Abstract:	We propose a method for marker-based selection of cultivars of clonally-reproducing plant species which keeps the basic genetic architecture of a top-performing cultivar (usually a partly heterozygous genotype), with the addition of some agronomically relevant differences (such as production time, product appearance or quality), providing added value to the product or cultivation process. The method is based on selecting a) two complementary nearly-inbred lines from successive selfing generations (ideally only F₂ and F₃) of large size, that may generate individuals with most of their genome identical to the original cultivar but being homozygous for either of the two component haplotypes in the rest, and b) individuals with such characteristics already occurring in the F₂. Option a) allows for introgressing genes from other individuals in one or both of these nearly-inbred lines. Peach, a woody-perennial, clonally-reproduced species, was chosen as a model for a proof of concept of the Resynthesis process due to its biological characteristics: self-compatibility, compact and genetically well-known genome, low recombination rates and relatively short intergeneration time (3-4 years). From 416 F₂ seedlings from cultivar Sweet Dream (SD), we obtained seven individuals with 76-94% identity with SD, and selected five pairs of complementary lines with average homozygosity of the two parents ≥0. 70 such that crossing would produce some individuals highly similar to SD. The application of this scheme to other species with more complex genomes or biological features, including its generalization to F₁ hybrids, is discussed.
Grants:	Ministerio de Economía y Competitividad AGL2012-40228-C02-01 Ministerio de Economía y Competitividad RTA2015-00050-00-00 Ministerio de Economía y Competitividad SEV-2015-0533
Note:	Altres ajuts: CERCA Programme/Generalitat de Catalunya
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Breeding methods ; Complementary lines ; Nearly-inbred lines ; Gene introgression ; Vegetatively-reproducing crops ; Peach ; F1 hybrids
Published in:	Frontiers in plant science, Vol. 11 (August 2020) , art. 1205, ISSN 1664-462X

DOI: 10.3389/fpls.2020.01205
PMID: 32849747

8 p, 813.1 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles