Identification of a new allele of the Dw gene causing brachytic dwarfing in peach
Cantin, Celia M. (Institut de Recerca i Tecnologia Agroalimentàries. Parc Científic i Tecnològic Agroalimentari de Lleida)
Arús i Gorina, Pere (Centre de Recerca en Agrigenòmica)
Eduardo, Iban (Centre de Recerca en Agrigenòmica)

Date:	2018
Abstract:	Objective: Peach brachytic dwarfism determined by Dwarf gene (Dw) is an undesired trait segregating in some peach breeding programs. Recently, a single nucleotide polymorphism (SNP) mutation in the gibberellin insensitive dwarf 1 (GID1) peach gene causing brachytic dwarfism was described. In this research we wanted to validate this marker in an F2 population of the 'Nectavantop' peach cultivar (Nv) to include it as a marker assisted selection tool for peach breeding programs. Results: The observed segregation of the trait was in agreement with that of a recessive gene, the individuals homozygous for the recessive allele (dwdw) presenting the dwarf genotype. Dw was mapped to the distal part of linkage group 6 as previously described. The SNP marker based on the causal mutation previously described did not segregate in Nv F2 population. The sequence of the GID1c gene in Nv revealed a second SNP in its coding sequence which cosegregated with the dwarf phenotype. This SNP was predicted by the SNAP2 software to cause a major functional change and was validated in the dwarf peach cultivar 'Small sunning'. These results suggest the existence of at least two independent mutations of the Dw gene causing the peach brachytic dwarf phenotype.
Grants:	Ministerio de Economía y Competitividad SEV-2015-0533 Ministerio de Economía y Competitividad AGL2012-40228
Note:	Altres ajuts: CERCA Programme/Generalitat de Catalunya
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Tree architecture ; Molecular marker ; Prunus persica ; Marker-assisted selection
Published in:	BMC research notes, Vol. 11 (June 2018) , art. 386, ISSN 1756-0500

DOI: 10.1186/s13104-018-3490-7
PMID: 29898773

5 p, 1.3 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles