Paper-based electrophoretic bioassay : biosensing in whole blood operating via smartphone
Sena Torralba, Amadeo (Institut Català de Nanociència i Nanotecnologia)
Álvarez Diduk, Ruslan (Institut Català de Nanociència i Nanotecnologia)
Parolo, Claudio (Institut Català de Nanociència i Nanotecnologia)
Torné-Morató, Helena (Institut Català de Nanociència i Nanotecnologia)
Müller, Alexander (Institut Català de Nanociència i Nanotecnologia)
Merkoçi, Arben (Institut Català de Nanociència i Nanotecnologia)

Date:	2021
Abstract:	Point-of-care (PoC) tests are practical and effective diagnostic solutions for major clinical problems, ranging from the monitoring of a pandemic to recurrent or simple measurements. Although, in recent years, a great improvement in the analytical performance of such sensors has been observed, there is still a major issue that has not been properly solved: The ability to perform adequate sample treatments. The main reason is that normally sample treatments require complicated or long procedures not adequate for deployment at the PoC. In response, a sensing platform, called paperbased electrophoretic bioassay (PEB), that combines the key characteristics of a lateral flow assay (LFA) with the sample treatment capabilities of electrophoresis is developed. In particular, the ability of PEB to separate different types of particles and to detect human antibodies in untreated spiked whole blood is demonstrated. Finally, to make the platform suitable for PoC, PEB is coupled with a smartphone that controls the electrophoresis and reads the optical signal generated. It is believed that the PEB platform represents a much-needed solution for the detection of low target concentrations in complex media, solving one of the major limitations of LFA and opening opportunities for point-of-care sensors.
Grants:	European Commission 795635 Ministerio de Economía y Competitividad PCIN-2016-066 Ministerio de Economía y Competitividad MAT2017-87202-P Ministerio de Economía y Competitividad SEV2201320295
Note:	Altres ajuts: this work is also funded by the CERCA Program/Generalitat de Catalunya.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Analytical performance ; Clinical problems ; Human antibodies ; Key characteristics ; Lateral flow assay ; Sample treatment ; Sensing platforms ; Target concentrations
Published in:	Analytical chemistry, Vol. 93, issue 6 (Feb. 2021) , p. 3112-3121, ISSN 1520-6882

DOI: 10.1021/acs.analchem.0c04330

10 p, 2.8 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles