Neurohormonal activation induces intracellular iron deficiency and mitochondrial dysfunction in cardiac cells
Tajes, Marta (Hospital Universitari de Bellvitge)
Díez-López, C. (Universitat de Barcelona)
Enjuanes, C.. (Hospital Universitari de Bellvitge)
Moliner, P. (Hospital Universitari de Bellvitge)
Ferreiro, J. L. (Hospital Universitari de Bellvitge)
Garay, Alberto (Hospital Universitari de Bellvitge)
Jiménez-Marrero, S. (Hospital Universitari de Bellvitge)
Yun, Sergi (Hospital Universitari de Bellvitge)
Sosa, S. G. (Hospital Universitari de Bellvitge)
Alcoberro, L. (Hospital Universitari de Bellvitge)
Gonzalez-Costello, Jose (Universitat de Barcelona)
García-Romero, E. (Hospital Universitari de Bellvitge)
Yañez-Bisbe, L. (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Benito, B. (Universitat Autònoma de Barcelona. Departament de Medicina)
Comín-Colet, Josep (Universitat de Barcelona. Departament de Ciències Clíniques)

Date:	2021
Abstract:	Iron deficiency (ID) is common in patients with heart failure (HF) and is associated with poor outcomes, yet its role in the pathophysiology of HF is not well-defined. We sought to determine the consequences of HF neurohormonal activation in iron homeostasis and mitochondrial function in cardiac cells. HF was induced in C57BL/6 mice by using isoproterenol osmotic pumps and embryonic rat heart-derived H9c2 cells were subsequently challenged with Angiotensin II and/or Norepinephrine. The expression of several genes and proteins related to intracellular iron metabolism were assessed by Real time-PCR and immunoblotting, respectively. The intracellular iron levels were also determined. Mitochondrial function was analyzed by studying the mitochondrial membrane potential, the accumulation of radical oxygen species (ROS) and the adenosine triphosphate (ATP) production. Hearts from isoproterenol-stimulated mice showed a decreased in both mRNA and protein levels of iron regulatory proteins, transferrin receptor 1, ferroportin 1 and hepcidin compared to control mice. Furthermore, mitoferrin 2 and mitochondrial ferritin were also downregulated in the hearts from HF mice. Similar data regarding these key iron regulatory molecules were found in the H9c2 cells challenged with neurohormonal stimuli. Accordingly, a depletion of intracellular iron levels was found in the stimulated cells compared to non-stimulated cells, as well as in the hearts from the isoproterenol-induced HF mice. Finally, neurohormonal activation impaired mitochondrial function as indicated by the accumulation of ROS, the impaired mitochondrial membrane potential and the decrease in the ATP levels in the cardiac cells. HF characteristic neurohormonal activation induced changes in the regulation of key molecules involved in iron homeostasis, reduced intracellular iron levels and impaired mitochondrial function. The current results suggest that iron could be involved in the pathophysiology of HF.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Neurohormonal activation ; Heart failure ; Iron deficiency ; Cardiac cell ; Mitochondria function
Published in:	Cell & Bioscience, Vol. 11 (may 2021) , ISSN 2045-3701

DOI: 10.1186/s13578-021-00605-5
PMID: 34001233

18 p, 4.0 MB

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