New Sperm Morphology Analysis in Equids : Trumorph ® Vs Eosin-Nigrosin Stain
Gacem, Sabrina (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Catalán, Jaime (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Yánez-Ortiz, Iván (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Soler, Carles (Universitat de València)
Miró, Jordi (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)

Data:	2021
Resum:	The evaluation of the male fertility potential is based on the analysis of the basic spermatic characteristics of concentration, motility and morphology. Thus, the study of sperm morphology is a fundamental element in the seminal analysis, but its real meaning has been biased by the techniques used for its evaluation. These techniques involve dehydration phases and subsequent staining, which involves the production of artifacts. The aim of the study is to compare two methods for equid semen morphology evaluation, Trumorph ® using living sperm vs. eosin-nigrosine stain. A total of 49 ejaculates from stallions and donkeys were used. After semen collection and dilution, an aliquot was placed on the slide and introduced in the Trumorph ® device. Then observation was made with a 40x objective and negative phase-contrast microscope. Another aliquot was stained using eosin-nigrosine stain and viewed using 100× magnification. Well-formed sperm were observed, and different abnormalities were identified using Trumorph ®. The use of eosin-nigrosin staining method and Trumorph ® led to the same results and both techniques can be used for stallion and donkey sperm morphological analysis. However, considering the fact that Trumorph ® uses living sperm helps prevent sperm cell alteration during sample preparation. Therefore, Trumorph ® can be a good alternative to the conventional staining method, which provides a quick test on live sperm.
Nota:	Equine Reproduction Service. Department of Animal Medicine and Surgery. Veterinary Faculty. Autonomous University of Barcelona. National Agency for Research and Development (ANID), Ministry of Education, Chile (2017/72180128). Secretary of Higher Education, Science, Technology and Innovation (SENESCYT)(CZ02-000507-2019)
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Ases ; Ases -- Espermatozoides ; Horse ; Donkey ; Sperm morphology ; Eosin-nigrosin stain ; Trumorph ®
Publicat a:	Veterinary Sciences, Vol. 8 (may 2021) , ISSN 2306-7381

DOI: 10.3390/vetsci8050079
PMID: 34066550

9 p, 4.4 MB

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