Sustainable natural bioresources in crop protection : antimicrobial hydroxycoumarins induce membrane depolarization-associated changes in the transcriptome of Ralstonia solanacearum
Yang, Liang (Centre de Recerca en Agrigenòmica)
Guan, Dailu (Centre de Recerca en Agrigenòmica)
Valls, Marc (Centre de Recerca en Agrigenòmica)
Ding, Wei (Southwest University. College of Plant Protection. Laboratory of Natural Products Pesticides (China))

Date:	2021
Abstract:	BACKGROUND: Ralstonia solanacearum is one of the most devastating pathogen affect crop production worldwide. Hydroxycoumarin (umbelliferone, esculetin, daphnetin) imply as sustainable natural bioresources on controlling of plant bacterial wilt. However, the antibacterial mechanism of hydroxycoumarins against plant pathogen still remains poorly understood. RESULTS: Here we characterized the effect of three hydroxycoumarins on the transcriptome of R. solanacearum. All three hydroxycoumarins were able to kill R. solanacearum, but their antibacterial activity impacted differently the bacterial transcriptome, indicating that their modes of action might be different. Treatment of R. solanacearum cultures with hydroxycoumarins resulted in a large number of the differentially expressed genes (DEGs), involved in basic cellular functions and metabolic process, such as downregulation of genes involved in fatty acid synthesis, lipopolysaccharides biosynthesis, RNA modification, ribosomal submits, oxidative phosphorylation and electrontransport, as well as upregulation of genes involved in transcriptional regulators, drug efflux, and oxidative stress responses. Future studies based on in vitro experiments are proposed to investigate lipopolysaccharides biosynthesis pathway leading to R. solanacearum cell death caused by hydroxycoumarins. Deletion of lpxB substantially inhibited the growth of R. solanacearum, and reduced virulence of pathogen on tobacco plants. CONCULSION: Our transcriptomic analyses show that hydroxycoumarins specific suppressed genes expression involved in fatty acid synthesis, RNA modification, ribosomal submits, oxidative phosphorylation and electrontransport. These findings provide evidence that hydroxycoumarins inhibit R. solanacearum growth through affect multi-target effect. Hydroxycoumarins could serve as sustainable natural bioresources against plant bacterial wilt through membrane destruction targeting the lipopolysaccharides biosynthesis pathway.
Rights:	Tots els drets reservats.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	Transcriptome analysis ; R. solanacearum ; Hydroxycoumarins ; Antibacterial activity ; Membrane
Published in:	Pest Management Science, Vol. 77, Issue 11 (November 2021) , p. 5170-5185, ISSN 1526-4998

DOI: 10.1002/ps.6557

Postprint 43 p, 4.0 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles