Molecular detection of Ralstonia solanacearum to facilitate breeding for resistance to bacterial wilt in potato
Ferreira, Virginia (Universidad de la República. Área Microbiología (Uruguay))
González, Matías (Instituto Nacional de Investigaciones Agropecuarias (Uruguay). Estación Experimental Salto Grande)
Pianzzola, María Julia (Universidad de la República. Área Microbiología (Uruguay))
Sánchez Coll, Núria (Centre de Recerca en Agrigenòmica)
Siri, María Inés (Universidad de la República. Área Microbiología (Uruguay))
Valls, Marc (Centre de Recerca en Agrigenòmica)

Publicación:	New York : Humana, 2021
Descripción:	11 pàg.
Resumen:	Potato bacterial wilt is caused by the devastating bacterial pathogen Ralstonia solanacearum. Quantitative resistance to this disease has been and is currently introgressed from a number of wild relatives into cultivated varieties through laborious breeding programs. Here, we present two methods that we have developed to facilitate the screening for resistance to bacterial wilt in potato. The first one uses R. solanacearum reporter strains constitutively expressing the luxCDABE operon or the green fluorescent protein (gfp) to follow pathogen colonization in potato germplasm. Luminescent strains are used for nondestructive live imaging, while fluorescent ones enable precise pathogen visualization inside the plant tissues through confocal microscopy. The second method is a BIO-multiplex-PCR assay that is useful for sensitive and specific detection of viable R. solanacearum (IIB-1) cells in latently infected potato plants. This BIO-multiplex-PCR assay can specifically detect IIB-1 sequevar strains as well as strains belonging to all four R. solanacearum phylotypes and is sensitive enough to detect without DNA extraction ten bacterial cells per mL in complex samples. The described methods allow the detection of latent infections in roots and stems of asymptomatic plants and were shown to be efficient tools to assist potato breeding programs.
Derechos:	Tots els drets reservats.
Lengua:	Anglès
Colección:	Methods in Molecular Biology ; 2354
Documento:	Capítol de llibre ; Versió acceptada per publicar
Publicado en:	Solanum tuberosum, 2021, p. 375-385, ISBN 978-1-0716-1609-3

DOI: 10.1007/978-1-0716-1609-3_18

Postprint 17 p, 554.6 KB

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