Assessment of aerobic biodegradation of lower-chlorinated benzenes in contaminated groundwater using field-derived microcosms and compound-specific carbon isotope fractionation
Trueba-Santiso, Alba (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Palau Capdevila, Jordi (Universitat de Barcelona. Departament de Mineralogia)
Soder-Walz, Jesica M. (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Vicent i Huguet, Teresa (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Marco Urrea, Ernest (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)

Data:	2022
Resum:	Biodegradation of lower chlorinated benzenes (tri-, di- and monochlorobenzene) was assessed at a coastal aquifer contaminated with multiple chlorinated aromatic hydrocarbons. Field-derived microcosms, established with groundwater from the source zone and amended with a mixture of lower chlorinated benzenes, evidenced biodegradation of monochlorobenzene (MCB) and 1,4-dichlorobenzene (1,4-DCB) in aerobic microcosms, whereas the addition of lactate in anaerobic microcosms did not enhance anaerobic reductive dechlorination. Aerobic microcosms established with groundwater from the plume consumed several doses of MCB and concomitantly degraded the three isomers of dichlorobenzene with no observable inhibitory effect. In the light of these results, we assessed the applicability of compound stable isotope analysis to monitor a potential aerobic remediation treatment of MCB and 1,4-DCB in this site. The carbon isotopic fractionation factors (ε) obtained from field-derived microcosms were -0. 7 ± 0. 1 ‰ and -1. 0 ± 0. 2 ‰ for MCB and 1,4-DCB, respectively. For 1,4-DCB, the carbon isotope fractionation during aerobic biodegradation was reported for the first time. The weak carbon isotope fractionation values for the aerobic pathway would only allow tracing of in situ degradation in aquifer parts with high extent of biodegradation. However, based on the carbon isotope effects measured in this and previous studies, relatively high carbon isotope shifts (i. e. , ∆δ13C > 4. 0 ‰) of MCB or 1,4-DCB in contaminated groundwater would suggest that their biodegradation is controlled by anaerobic reductive dechlorination.
Ajuts:	Ministerio de Economía y Competitividad CGL2017-82331-R Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-1733
Nota:	Altres ajuts: acords transformatius de la UAB
Nota:	Altres ajuts: Agència catalana de l'Aigua CTN1900901
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Groundwater ; Isotope fractionation ; Chlorobenzenes ; Aerobic biodegradation ; Anaerobic reductive dehalogenation ; Natural attenuation
Publicat a:	Journal of environmental sciences, Vol. 118 (August 2022) , p. 204-213, ISSN 1878-7320

DOI: 10.1016/j.jes.2021.12.025
PMID: 35305769

10 p, 1.0 MB

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