Tween 80 Improves the Acid-Fast Bacilli Quantification in the Magnetic Nanoparticle-Based Colorimetric Biosensing Assay (NCBA)
Gordillo-Marroquín, Cristina (Social Observatory of Tuberculosis Mexico, El Colegio de La Frontera Sur (ECOSUR))
Sánchez-Pérez, Héctor J. (Social Observatory of Tuberculosis Mexico, El Colegio de La Frontera Sur (ECOSUR))
Gómez-Velasco, Anaximandro (Department of Human Ecology, Center for Research and Advanced Studies of the National Polytechnic Institute (Cinvestav-IPN))
Martín Mateo, Miguel (Universitat Autònoma de Barcelona. Departament de Pediatria, Obstetrícia i Ginecologia i Medicina Preventiva i Salut Pública)
Guillén-Navarro, Karina (Sustainability Sciences Department, El Colegio de la Frontera Sur (ECOSUR))
Vázquez-Marcelín, Janeth (Mycobacteriology Laboratory, TB Prevention and Control Program for the Highlands of Chiapas, Ministry of Health of Chiapas)
Gómez-Bustamante, Adriana (State Public Health Laboratory for Chiapas, Ministry of Health of Chiapas)
Jonapá-Gómez, Letisia (State Public Health Laboratory for Chiapas, Ministry of Health of Chiapas)
Alocilja, Evangelyn C. (Nano-Biosensors Laboratory, Department of Biosystems and Agricultural Engineering, Michigan State University)

Data:	2022
Resum:	Despite its reduced sensitivity, sputum smear microscopy (SSM) remains the main diagnostic test for detecting tuberculosis in many parts of the world. A new diagnostic technique, the magnetic nanoparticle-based colorimetric biosensing assay (NCBA) was optimized by evaluating different concentrations of glycan-functionalized magnetic nanoparticles (GMNP) and Tween 80 to improve the acid-fast bacilli (AFB) count. Comparative analysis was performed on 225 sputum smears: 30 with SSM, 107 with NCBA at different GMNP concentrations, and 88 with NCBA-Tween 80 at various concentrations and incubation times. AFB quantification was performed by adding the total number of AFB in all fields per smear and classified according to standard guidelines (scanty, 1+, 2+ and 3+). Smears by NCBA with low GMNP concentrations (≤1. 5 mg/mL) showed higher AFB quantification compared to SSM. Cell enrichment of sputum samples by combining NCBA-GMNP, incubated with Tween 80 (5%) for three minutes, improved capture efficiency and increased AFB detection up to 445% over SSM. NCBA with Tween 80 offers the opportunity to improve TB diagnostics, mainly in paucibacillary cases. As this method provides biosafety with a simple and inexpensive methodology that obtains results in a short time, it might be considered as a point-of-care TB diagnostic method in regions where resources are limited.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Tuberculosis ; Mycobacterium tuberculosis ; Tb detection ; Nanotechnology ; Sputum smear microscopy ; Tween 80 ; Paucibacillary samples ; Increased sensitivity ; Diagnostic methods
Publicat a:	Biosensors, Vol. 12 Num. 1(january 2022) , ISSN 2079-6374

DOI: 10.3390/bios12010029
PMID: 35049656

18 p, 5.4 MB

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