A Longitudinal Analysis Reveals Early Activation and Late Alterations in B Cells During Primary HIV Infection in Mozambican Adults
Jiménez, Montse (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Pastor Palomo, Lucía (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Urrea, Víctor (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Rodríguez de la Concepción, María Luisa (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Parker, Erica (Faculty of Health and Medical Sciences, University of Western Australia)
Fuente-Soro, Laura (Centro de Investigação em Saúde da Manhiça (CISM))
Jairoce, Chenjerai (Centro de Investigação em Saúde da Manhiça (CISM))
Mandomando, Inacio (Centro de Investigação em Saúde da Manhiça (CISM))
Carrillo, Jorge (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Naniche, Denise (Centro de Investigação em Saúde da Manhiça (CISM))
Blanco, Julià (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Universitat Autònoma de Barcelona

Fecha:	2021
Resumen:	Primary HIV infection (PHI) and subsequent chronic infection alter B-cell compartment. However, longitudinal analysis defining the dynamics of B-cell alterations are still limited. We longitudinally studied B-cell subsets in individuals followed for 1 year after PHI (n = 40). Treated and untreated chronic HIV infected (n = 56) and HIV-uninfected individuals (n = 58) were recruited as reference groups at the Manhiça District in Mozambique. B cells were analyzed by multicolor flow-cytometry. Anti-HIV humoral response and plasma cytokines were assessed by ELISA or Luminex-based technology. A generalized activation of B cells induced by HIV occurs early after infection and is characterized by increases in Activated and Tissue-like memory cells, decreases in IgM-IgD- (switched) and IgM-only B cells. These alterations remain mostly stable until chronic infection and are reverted in part by ART. In contrast, other parameters followed particular dynamics: PD-1 expression in memory cells decreases progressively during the first year of infection, Transitional B cells expand at month 3-4 after infection, and Marginal zone-like B cells show a late depletion. Plasmablasts expand 2 months after infection linked to plasma viral load and anti-p24 IgG3 responses. Most of well-defined changes induced by HIV in B-cell activation and memory subsets are readily observed after PHI, lasting until ART initiation. However, subsequent changes occur after sustained viral infection. These data indicate that HIV infection impacts B cells in several waves over time, and highlight that early treatment would result in beneficial effects on the B-cell compartment.
Ayudas:	Ministerio de Ciencia e Innovación 2011-27901 Instituto de Salud Carlos III DTS15/00185 Instituto de Salud Carlos III FI12/00096
Nota:	Altres ajuts: Melinda Gates Foundation (OPP1068252)
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	B-cell activation ; Plasmablasts ; Marginal zone (MZ) B cell ; Transitional B cell ; PD-1
Publicado en:	Frontiers in immunology, Vol. 11 (january 2021) , ISSN 1664-3224

DOI: 10.3389/fimmu.2020.614319
PMID: 33519823

12 p, 1.4 MB

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