Quantification of parasite burden of Trypanosoma cruzi and identification of Discrete Typing Units (DTUs) in blood samples of Latin American immigrants residing in Barcelona, Spain
Tavares de Oliveira, Maykon (Hospital Universitari Vall d'Hebron)
Sulleiro Igual, Elena (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Silgado, Aroa (Hospital Universitari Vall d'Hebron. Institut de Recerca)
de Lana, Marta (Federal University of Ouro Preto)
Zingales, Bianca (University of São Paulo)
Santana da Silva, João (University of São Paulo)
Marin-Neto, J. Antônio (University of São Paulo)
Molina Romero, Israel (Hospital Universitari Vall d'Hebron)
Universitat Autònoma de Barcelona

Data:	2020
Resum:	Trypanosoma cruzi has a high genetic and biological diversity and has been subdivided into seven genetic lineages, named TcI-TcVI and TcBat. DTUs TcI-TcII-TcV and TcVI are agents of ChD in different regions of Latin America. Due to population movements, the disease is an emergent global public health problem. Thus, the aim of this study was to quantify the parasitic load and identify the presence of T. cruzi DTUs in 101 Latin American immigrants with chronic ChD, residing in Barcelona, Spain. 5ml of peripheral blood were collected in guanidine/EDTA from each patient for DNA extraction, quantification of the parasitic load and genotyping. A great variation of the parasitic load of the patients was verified: from 0. 001 to 22. 2 T. cruzi DNA (fg) / Blood DNA (ng). In patients from Bolivia the parasitic load was 3. 76±4. 43 T. cruzi DNA (fg) / Blood DNA (ng) (mean ± SD), in patients of other countries was 0. 95±1. 38 T. cruzi DNA (fg) / Blood DNA (ng). No statistically significant difference was observed in the parasitic load between patients with the indeterminate and cardiac forms of ChD (p = 0,57). Parasite genotyping was performed by multilocus conventional PCR. In patients from Bolivia there was a nearly equal prevalence of DTUs TcV (27/77), TcII/TcV/TcVI (26/77), and TcII/TcVI (22/77). TcVI was detected in only 2 samples (2/77). A higher prevalence of TcII/TcVI (19/24) was verified in patients of other countries, with low prevalence of TcII/TcV/TcVI (4/24) and TcV (1/24). In this study, low/medium parasitic load was found in all patients evaluated. Our data corroborate previous conclusions indicating that patients from the Bolivia, living in Spain, are predominantly infected by TcV, and TcVI DTUs. On the other hand, in Non-Bolivians patients TcII/TcVI predominated. Surprisingly, in our cohort of 101 patients no infection by TcI DTU was observed. Trypanosoma cruzi is divided in seven distinct genetic groups (TcI-TcVI) and TcBat. They can be related to several biological parameters, the main being resistance to specific treatment. Due to the intense migration movements, ChD has become a serious public health problem in Europe. Thus, the work has the important function of identifying the genetic variability of T. cruzi circulating in the European continent, in addition to assessing the parasitic burden present in 101 chronic chagasic patients, residing in Barcelona, Spain. We show differences in the predominance between the infecting DTUs among Bolivian (TcV) and non-Bolivian patients (TcII/TcVI). This is the first study to describe the presence of TcVI genotype in Europe. Although the level of parasite burden is low/medium, it is higher in patients from Bolivia when compared with patients of other countries. The low parasitic burden is a limitation factor for studies aimed at evaluating by qPCR the effects of treating this disease with the drugs available to date, Benznidazole and Nifurtimox, and for clinical trials of new drugs. The information generated in this study should impact planning of more effective public health interventions to improve the health of chagasic patients, control vertical transmission and treatment of ChD.
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Llengua:	Angles
Document:	Article ; Versió publicada
Publicat a:	PLoS neglected tropical diseases, Vol. 14 (june 2020) , ISSN 1935-2735

DOI: 10.1371/journal.pntd.0008311
PMID: 32497037

14 p, 1.1 MB

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