Evaluation of P22 Antigenic Complex for the Immuno-Diagnosis of Tuberculosis in BCG Vaccinated and Unvaccinated Goats
Arrieta-Villegas, Claudia (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Infantes-Lorenzo, José Antonio (Centro Nacional de Microbiología (Espanya))
Bezos, Javier (Universidad Complutense de Madrid. Departamento de Sanidad Animal)
Grasa, Miriam (Agrupació de Defensa Sanitària de Cabrum i Oví Lleter de Catalunya)
Vidal Barba, Enric (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Mercader, Irene (Generalitat de Catalunya. Departament d'Agricultura, Ramaderia, Pesca i Alimentació (DARP))
Singh, Mahavir (LIONEX Diagnostics and Therapeutics)
Domingo, Mariano (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
De Juan Ferré, Lucía (Universidad Complutense de Madrid. Departamento de Sanidad Animal)
Pérez de Val, Bernat (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)

Data:	2020
Resum:	Current eradication strategies of tuberculosis (TB) in goats mainly rely on the single intradermal tuberculin test (SIT) and single intradermal cervical comparative tuberculin tests (SICCTs). TB vaccination has been proposed as a cost-effective option in high-prevalence herds or countries where economic compensation for the slaughter of positive animals is not affordable. However, TB vaccination compromises the efficiency of tuberculin-based diagnostic tests. In this study, the performance of a new diagnostic platform, based on the P22 antigenic complex, was assessed for skin test (ST), interferon-gamma release assay (IGRA), and serology under different TB scenarios. The sensitivity (Se) of diagnostic tests was assessed in TB-infected goats from the same farm (herd A, N = 77). The specificity (Sp) was assessed in two TB-negative farms (both vaccinated against paratuberculosis): one TB unvaccinated (herd B, N = 77) and another vaccinated with bacille Calmette-Guérin (BCG) (herd C, N = 68). The single (s) P22-IGRA showed the highest Se among IGRA tests (91%), and the comparative (c) P22-ST showed the highest Sp (100% in herd B and 98% in herd C). Combined interpretation of techniques enabled the best diagnostic performances. Combining the SICCT + sP22-IGRA improved Se (97%) compared to SICCT + tuberculin-based IGRA (95%), with a reduction of Sp (95 and 100%, respectively). Besides, combination of P22-ELISA with cP22-ST or SICCT elicited a similar performance in the non-vaccination context (Se: 94 and 95%; Sp: 95 and 95%, respectively), but Sp was significantly higher for the combination with cP22-ST compared to SICCT in the TB vaccination context (95 and 79%, respectively). The combination of serological tests based on P22 and MPB83 showed higher complementarity and improved 13 percentage points the Se of P22-ELISA alone. These findings suggest that either cell-mediated or antibody-based diagnostic techniques, using the P22 antigen complex, can contribute to improve the immunodiagnostics of TB in goats under different TB control strategies.
Ajuts:	Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria RTA2015-00043-C02-00 Ministerio de Economía y Competitividad FPI-INIA 2016: CPD2016-0109
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Tuberculosis ; Diagnosis ; Goats ; Bacille Calmette-Guérin (BCG) ; Skin test ; Interferon-gamma release assay (IGRA) ; Serology ; P22
Publicat a:	Frontiers in Veterinary Science, Vol. 7 (july 2020) , ISSN 2297-1769

DOI: 10.3389/fvets.2020.00374
PMID: 32714950

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