Sampling Device-Dependence of Prokaryotic Community Structure on Marine Particles : Higher Diversity Recovered by in situ Pumps Than by Oceanographic Bottles
Puigcorbé, Viena (Edith Cowan University. Centre for Marine Ecosystems Research)
Ruiz-González, Clara (Institut de Ciències del Mar. Departament de Biologia Marina i Oceanografia)
Masqué Barri, Pere (Universitat Autònoma de Barcelona. Institut de Ciència i Tecnologia Ambientals)
Gasol, Josep M. (Institut de Ciències del Mar. Departament de Biologia Marina i Oceanografia)

Data:	2020
Resum:	Microbes associated with sinking marine particles play key roles in carbon sequestration in the ocean. The sampling of particle-attached microorganisms is often done with sediment traps or by filtration of water collected with oceanographic bottles, both involving a certain time lapse between collection and processing of samples that may result in changes in particle-attached microbial communities. Conversely, in situ water filtration through submersible pumps allows a faster storage of sampled particles, but it has rarely been used to study the associated microbial communities and has never been compared to other particle-sampling methods in terms of the recovery of particle microbial diversity. Here we compared the prokaryotic communities attached to small (1-53 μm) and large (>53 μm) particles collected from the mesopelagic zone (100-300 m) of two Antarctic polynyas using in situ pumps (ISP) and oceanographic bottles (BTL). Each sampling method retrieved largely different particle-attached communities, suggesting that they capture different kinds of particles. These device-driven differences were greater for large particles than for small particles. Overall, the ISP recovered 1. 5- to 3-fold more particle-attached bacterial taxa than the BTL, and different taxonomic groups were preferentially recovered by each method. In particular, typical particle-attached groups such as Planctomycetes and Deltaproteobacteria recovered with ISP were nearly absent from BTL samples. Our results suggest that the method used to sample marine particles has a strong influence in our view of their associated microbial communities.
Ajuts:	Ministerio de Economía y Competitividad CTM2015-70340-R Agencia Estatal de Investigación RTI2018-101025-B-I00 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-1568 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-1588 Ministerio de Economía y Competitividad MDM2015-0552
Nota:	Unidad de excelencia María de Maeztu CEX2019-000940-M
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Prokaryotic communities ; Marine particles ; Size-fractionation ; In situ pumps ; Oceanographic bottles ; Polynyas
Publicat a:	Frontiers in microbiology, Vol. 11 (July 2020) , art. 1615, ISSN 1664-302X

DOI: 10.3389/fmicb.2020.01645
PMID: 32760385

10 p, 1.2 MB

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