Beneficial Effects of Glucagon-Like Peptide-1 (GLP-1) in Diabetes-Induced Retinal Abnormalities : Involvement of Oxidative Stress
Ramos, Hugo (Hospital Universitari Vall d'Hebron)
Bogdanov, Patricia (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Sampedro, Joel (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Huerta, Jordi (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Simó Canonge, Rafael (Universitat Autònoma de Barcelona. Departament de Medicina)
Hernández, Cristina (Universitat Autònoma de Barcelona. Departament de Medicina)

Date:	2020
Abstract:	Background: Hyperglycemia-induced oxidative stress plays a key role in diabetic complications, including diabetic retinopathy. The main goal of this study was to assess whether the topical administration (eye drops) of glucagon-like peptide-1 (GLP-1) has any effect on oxidative stress in the retina. Methods: db/db mice were treated with eye drops of GLP-1 or vehicle for three weeks, with db/+ mice being used as control. Studies included the assessment by western blot of the antioxidant defense markers CuZnSOD, MnSOD, glutathione peroxidase and reductase; immunofluorescence measurements of DNA/RNA damage, nitro tyrosine and Ki67 and Babam2 proteins. Results: GLP-1 eye drops protected from oxidative stress by increasing the protein levels of glutathione reductase, glutathione peroxidase and CuZnSOD and MnSOD in diabetic retinas. This was associated with a significant reduction of DNA/RNA damage and the activation of proteins involved in DNA repair in the retina (Babam2) and Ki67 (a biomarker of cell proliferation). Conclusions: GLP-1 modulates the antioxidant defense system in the diabetic retina and has a neuroprotective action favoring DNA repair and neuron cells proliferation.
Grants:	Ministerio de Economía y Competitividad BES-2017-081690 Ministerio de Economía y Competitividad DTS15/00151 Ministerio de Economía y Competitividad PI16/00541 Ministerio de Economía y Competitividad SAF2016-77784
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Diabetic retinopathy ; GLP-1 ; Oxidative stress ; Superoxide dismutase ; Free radicals
Published in:	Antioxidants, Vol. 9 (september 2020) , ISSN 2076-3921

DOI: 10.3390/antiox9090846
PMID: 32927585

12 p, 4.0 MB

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