Potential of Extracellular Vesicle-Associated TSG-6 from Adipose Mesenchymal Stromal Cells in Traumatic Brain Injury
Roura, Santiago (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Monguió-Tortajada, Marta (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Munizaga-Larroudé, Micaela (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Clos Sansalvador, Marta (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Franquesa, Marcella (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Rosell Novel, Anna (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Borràs i Serres, Francesc Enric (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)

Fecha:	2020
Resumen:	Multipotent mesenchymal stromal cells (MSC) represent a promising strategy for a variety of medical applications. Although only a limited number of MSC engraft and survive after in vivo cellular infusion, MSC have shown beneficial effects on immunomodulation and tissue repair. This indicates that the contribution of MSC exists in paracrine signaling, rather than a cell-contact effect of MSC. In this review, we focus on current knowledge about tumor necrosis factor (TNF)-stimulated gene-6 (TSG-6) and mechanisms based on extracellular vesicles (EV) that govern long-lasting immunosuppressive and regenerative activity of MSC. In this context, in particular, we discuss the very robust set of findings by Jha and colleagues, and the opportunity to potentially extend their research focus on EV isolated in concentrated conditioned media (CCM) from adipose tissue derived MSC (ASC). Particularly, the authors showed that ASC-CCM mitigated visual deficits after mild traumatic brain injury in mice. TSG-6 knockdown ASC were, then, used to generate TSG-6-depleted CCM that were not able to replicate the alleviation of abnormalities in injured animals. In light of the presented results, we envision that the infusion of much distilled ASC-CCM could enhance the alleviation of visual abnormalities. In terms of EV research, the advantages of using size-exclusion chromatography are also highlighted because of the enrichment of purer and well-defined EV preparations. Taken together, this could further delineate and boost the benefit of using MSC-based regenerative therapies in the context of forthcoming clinical research testing in diseases that disrupt immune system homeostasis.
Ayudas:	Agencia Estatal de Investigación SAF2017-84324-C2-1-R Agencia Estatal de Investigación PID2019-110137RB-I00 Instituto de Salud Carlos III PI17/00335 Instituto de Salud Carlos III PI17/01487 Instituto de Salud Carlos III PIC18/00014 Instituto de Salud Carlos III PI18/00256 Ministerio de Economía y Competitividad RD16/0009 Ministerio de Economía y Competitividad CB16/11/00403
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Conditioned medium ; Extracellular vesicles ; Immunomodulation ; Mesenchymal stromal cells ; Traumatic brain injury ; Paracrine function ; Regenerative medicine ; Tissue repair ; Translational medicine ; Tumor necrosis factor-stimulated gene-6
Publicado en:	International journal of molecular sciences, Vol. 21 (september 2020) , ISSN 1422-0067

DOI: 10.3390/ijms21186761
PMID: 32942629

21 p, 1020.6 KB

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Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
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