Changes in saliva analytes in equine acute abdominal disease : a sialochemistry approach
Contreras-Aguilar, M. D. (Universidad de Murcia. Laboratorio Interdisciplinario de Análisis Clínicos Interlab)
Escribano Tortosa, Damián (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Martínez-Subiela, Silvia (Universidad de Murcia. Laboratorio Interdisciplinario de Análisis Clínicos Interlab)
Martín-Cuervo, María (Universidad de Extremadura. Facultad de Veterinaria)
Lamy, Elsa (University of Évora. Institute of Mediterranean Agricultural and Environmental Sciences)
Tecles, Fernando (Universidad de Murcia. Laboratorio Interdisciplinario de Análisis Clínicos Interlab)
Cerón, José Joaquín (Universidad de Murcia. Laboratorio Interdisciplinario de Análisis Clínicos Interlab)

Fecha:	2019
Resumen:	The biochemical components of saliva can change in certain pathologies in horses, for example in acute abdominal disease. The aim of this study was (1) to evaluate if a panel of biochemical analytes usually used in serum can be measured in saliva of horses and (2) to study the possible changes of these biochemical analytes in saliva of horses affected by acute abdominal disease. A panel of 23 analytes was analytically validated in saliva of horses and possible changes in these analytes in a pilot study with six healthy horses and six horses with acute abdominal disease were evaluated. The analytes with significant changes were then evaluated in a larger population of 20 healthy and 37 diseased horses. Seven analytes showed significant increases in the pilot study which were confirmed in the larger population. The analytes which showed significant changes, and their median fold increase and significance shown in the larger population were salivary γ-glutamyl transferase (gGT, 2. 3 fold, P = 0. 001), creatine kinase (CK, 6. 2 fold, P < 0. 001), urea (2. 3 fold, P = 0. 001), total bilirubin (2. 6 fold, P < 0. 001), total proteins (3. 2 fold, P < 0. 001), phosphorus (P, 4. 5 fold, P < 0. 001) and alpha-amylase (sAA, 8. 5 fold, P < 0. 001). Total proteins, P and sAA showed sensitivities higher than 70% at their optimal cut-off points and a specificity of 100% in differentiating between healthy horses and those with acute abdominal disease. A panel of 23 biochemical analytes can be measured in saliva of horses, where gGT, CK, urea, total bilirubin, total protein, P and sAA levels are raised in horses with acute abdominal disease. The online version of this article (10. 1186/s12917-019-1933-6) contains supplementary material, which is available to authorized users.
Ayudas:	Ministerio de Economía y Competitividad R- 605/2016
Nota:	Altres ajuts: Fundación Séneca 19894/GERM/15. Universidad de Murcia: R- 605/2016. Portuguese Science Foundation IF/01778/2013
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Biomarkers ; Colic ; Horse ; Saliva ; Sialochemistry
Publicado en:	BMC veterinary research, Vol. 15 (june 2019) , ISSN 1746-6148

DOI: 10.1186/s12917-019-1933-6
PMID: 31170977

9 p, 746.4 KB

El registro aparece en las colecciones:
Artículos > Artículos de investigación
Artículos > Artículos publicados