Analysis of the neurotoxic effects of neuropathic organophosphorus compounds in adult zebrafish
Faria, Melissa (Institut de Diagnosi Ambiental i Estudis de l'Aigua)
Fuertes, Inmaculada (Institut de Diagnosi Ambiental i Estudis de l'Aigua)
Prats, Eva (CID-CSIC)
Abad, José Luis (Institut de Química Avançada de Catalunya)
Padrós, Francesc 1965- (Universitat Autònoma de Barcelona. Servei de Diagnòstic Patològic en Peixos)
Gómez-Canela, Cristian (Institut de Diagnosi Ambiental i Estudis de l'Aigua)
Casas, Josefina (Institut de Química Avançada de Catalunya)
Estevez, Jorge (University "Miguel Hernandez" of Elche. Institute of Bioengineering)
Vilanova, Eugenio (University "Miguel Hernandez" of Elche. Institute of Bioengineering)
Piña, Benjamin (Institut de Diagnosi Ambiental i Estudis de l'Aigua)
Raldúa, Demetrio (Institut de Diagnosi Ambiental i Estudis de l'Aigua)

Data:	2018
Resum:	Inhibition and aging of neuropathy target esterase (NTE) by exposure to neuropathic organophosphorus compounds (OPs) can result in OP-induced delayed neuropathy (OPIDN). In the present study we aimed to build a model of OPIDN in adult zebrafish. First, inhibition and aging of zebrafish NTE activity were characterized in the brain by using the prototypic neuropathic compounds cresyl saligenin phosphate (CBDP) and diisopropylphosphorofluoridate (DFP). Our results show that, as in other animal models, zebrafish NTE is inhibited and aged by both neuropathic OPs. Then, a neuropathic concentration inhibiting NTE activity by at least 70% for at least 24 h was selected for each compound to analyze changes in phosphatidylcholines (PCs), lysophosphatidylcholines (LPCs) and glycerolphosphocholine (GPC) profiles. In spite to the strong inhibition of the NTE activity found for both compounds, only a mild increase in the LPCs level was found after 48 h of the exposure to DFP, and no effect were observed by CBDP. Moreover, histopathological evaluation and motor function outcome analyses failed to find any neurological abnormalities in the exposed fish. Thus, our results strongly suggest that zebrafish is not a suitable species for the development of an experimental model of human OPIDN.
Ajuts:	Ministerio de Economía y Competitividad BES-2015-075023
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	Scientific reports, Vol. 8 (march 2018) , ISSN 2045-2322

DOI: 10.1038/s41598-018-22977-4
PMID: 29555973

14 p, 3.0 MB

El registre apareix a les col·leccions:
Articles > Articles de recerca
Articles > Articles publicats