Characterization of the rat cerebrospinal fluid proteome following acute cerebral ischemia using an aptamer-based proteomic technology
Simats Oriol, Alba (Hospital Universitari Vall d'Hebron. Institut de Recerca)
García-Berrocoso, Teresa (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Ramiro, Laura (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Giralt, Dolors (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Gill, Natalia (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Penalba, Anna (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Bustamante, Alejandro (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Rosell Novel, Anna (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Montaner, Joan (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Universitat Autònoma de Barcelona

Fecha:	2018
Resumen:	The limited accessibility to the brain has turned the cerebrospinal fluid (CSF) into a valuable source that may contribute to the complete understanding of the stroke pathophysiology. Here we have described the CSF proteome in the hyper-acute phase of cerebral ischemia by performing an aptamer-based proteomic assay (SOMAscan) in CSF samples collected before and 30 min after male Wistar rats had undergone a 90 min Middle Cerebral Artery Occlusion (MCAO) or sham-surgery. Proteomic results indicated that cerebral ischemia acutely increased the CSF levels of 716 proteins, mostly overrepresented in leukocyte chemotaxis and neuronal death processes. Seven promising candidates were further evaluated in rat plasma and brain (CKB, CaMK2A, CaMK2B, CaMK2D, PDXP, AREG, CMPK). The 3 CaMK2 family-members and CMPK early decreased in the infarcted brain area and, together with AREG, co-localized with neurons. Conversely, CKB levels remained consistent after the insult and specifically matched with astrocytes. Further exploration of these candidates in human plasma revealed the potential of CKB and CMPK to diagnose stroke, while CaMK2B and CMPK resulted feasible biomarkers of functional stroke outcome. Our findings provided insights into the CSF proteome following cerebral ischemia and identified new outstanding proteins that might be further considered as potential biomarkers of stroke.
Ayudas:	Agència de Gestió d'Ajuts Universitaris i de Recerca 2015 FI_B00952 Ministerio de Ciencia e Innovación R16/00008 Ministerio de Ciencia e Innovación CPII15/00003 Ministerio de Ciencia e Innovación PI15/00354 Ministerio de Ciencia e Innovación RD16/0019
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Publicado en:	Scientific reports, Vol. 8 (may 2018) , ISSN 2045-2322

DOI: 10.1038/s41598-018-26237-3
PMID: 29784938

14 p, 2.3 MB

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