Cells alter their tRNA abundance to selectively regulate protein synthesis during stress conditions
Torrent Burgas, Marc (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Chalancon, Guilhem (Medical Research Council. Laboratory of Molecular Biology)
Sánchez de Groot, Natalia (Medical Research Council. Laboratory of Molecular Biology)
Wuster, Arthur (Medical Research Council. Laboratory of Molecular Biology)
Babu, Madam M. (Medical Research Council. Laboratory of Molecular Biology)

Fecha:	2018
Resumen:	Decoding the information in mRNA during protein synthesis relies on tRNA adaptors, the abundance of which can affect the decoding rate and translation efficiency. To determine whether cells alter tRNA abundance to selectively regulate protein expression, we quantified changes in the abundance of individual tRNAs at different time points in response to diverse stress conditions in Saccharomyces cerevisiae. We found that the tRNA pool was dynamic and rearranged in a manner that facilitated selective translation of stress-related transcripts. Through genomic analysis of multiple data sets, stochastic simulations, and experiments with designed sequences of proteins with identical amino acids but altered codon usage, we showed that changes in tRNA abundance affected protein expression independently of factors such as mRNA abundance. We suggest that cells alter their tRNA abundance to selectively affect the translation rates of specific transcripts to increase the amounts of required proteins under diverse stress conditions.
Ayudas:	European Commission 330352 European Commission 299105 Ministerio de Economía y Competitividad SAF2014-56568-R Ministerio de Economía y Competitividad SAF2015-72518-EXP Ministerio de Economía y Competitividad RYC-2012-09999
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Publicado en:	Science Signaling, Vol. 11, Issue 546 (September 2018) , art. eaat6409, ISSN 1937-9145

DOI: 10.1126/scisignal.aat6409
PMID: 30181241

10 p, 1.6 MB

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