High-density multi-population consensus genetic linkage map for peach
Da Silva Linge, Cassia (Clemson University. Department of Plant and Environmental Sciences)
Antanaviciute, Laima (Clemson University. Department of Plant and Environmental Sciences)
Abdelghafar, Asma (Clemson University. Department of Plant and Environmental Sciences)
Arús i Gorina, Pere (Centre de Recerca en Agrigenòmica)
Bassi, Daniele (Università degli Studi di Milano. Dipartimento di Scienze Agrarie e Ambientali)
Rossini, Laura (Università degli Studi di Milano. Dipartimento di Scienze Agrarie e Ambientali)
Ficklin, Stephen (Washington State University, Department of Horticulture)
Gasic, Ksenija (Clemson University, Department of Plant and Environmental Sciences)

Fecha:	2018
Resumen:	Highly saturated genetic linkage maps are extremely helpful to breeders and are an essential prerequisite for many biological applications such as the identification of marker-trait associations, mapping quantitative trait loci (QTL), candidate gene identification, development of molecular markers for marker-assisted selection (MAS) and comparative genetic studies. Several high-density genetic maps, constructed using the 9K SNP peach array, are available for peach. However, each of these maps is based on a single mapping population and has limited use for QTL discovery and comparative studies. A consensus genetic linkage map developed from multiple populations provides not only a higher marker density and a greater genome coverage when compared to the individual maps, but also serves as a valuable tool for estimating genetic positions of unmapped markers. In this study, a previously developed linkage map from the cross between two peach cultivars 'Zin Dai' and 'Crimson Lady' (ZC 2) was improved by genotyping additional progenies. In addition, a peach consensus map was developed based on the combination of the improved ZC 2 genetic linkage map with three existing high-density genetic maps of peach and a reference map of Prunus. A total of 1,476 SNPs representing 351 unique marker positions were mapped across eight linkage groups on the ZC 2 genetic map. The ZC 2 linkage map spans 483. 3 cM with an average distance between markers of 1. 38 cM/marker. The MergeMap and LPmerge tools were used for the construction of a consensus map based on markers shared across five genetic linkage maps. The consensus linkage map contains a total of 3,092 molecular markers, consisting of 2,975 SNPs, 116 SSRs and 1 morphological marker associated with slow ripening in peach (SR). The consensus map provides valuable information on marker order and genetic position for QTL identification in peach and other genetic studies within Prunus and Rosaceae.
Ayudas:	Ministerio de Economía y Competitividad RTA2015-00050-00-00 Ministerio de Economía y Competitividad SEV-2015-0533
Nota:	Altres ajuts: CERCA Programme/Generalitat de Catalunya
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Publicado en:	PloS one, Vol. 13, Num. 11 (November 2018) , art. e0207724, ISSN 1932-6203

DOI: 10.1371/journal.pone.0207724
PMID: 30462743

16 p, 4.0 MB

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