Impact of probiotic Saccharomyces boulardii on the gut microbiome composition in HIV-treated patients : A double-blind, randomised, placebo-controlled trial
Villar, Judit (Institut Hospital del Mar d'Investigacions Mèdiques)
Güerri-Fernández, Robert (Universitat Autònoma de Barcelona. Departament de Medicina)
Moya, Andrés (Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública)
González, Alicia (Institut Hospital del Mar d'Investigacions Mèdiques)
Hernández, Juan J. (Reference Laboratory of Catalonia, Barcelona)
Lerma-Chippirraz, Elisabet (Institut Hospital del Mar d'Investigacions Mèdiques)
Guelar, Ana (Institut Hospital del Mar d'Investigacions Mèdiques)
Sorlí, Luisa (Institut Hospital del Mar d'Investigacions Mèdiques)
Horcajada, Juan Pablo (Universitat Autònoma de Barcelona. Departament de Medicina)
Artacho, Alejandro (Foundation for the Promotion of Health and Biomedical Research in the Valencian Community (FISABIO) and Cavanilles Institute of Biodiversity and Evolutionary Biology (Universitat de València))
D'Auria, Giuseppe (Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública)
Knobel Freud, Hernando (Universitat Autònoma de Barcelona. Departament de Medicina)

Data:	2017
Resum:	Dysbalance in gut microbiota has been linked to increased microbial translocation, leading to chronic inflammation in HIV-patients, even under effective HAART. Moreover, microbial translocation is associated with insufficient reconstitution of CD4+T cells, and contributes to the pathogenesis of immunologic non-response. In a double-blind, randomised, placebo-controlled trial, we recently showed that, compared to placebo, 12 weeks treatment with probiotic Saccharomyces boulardii significantly reduced plasma levels of bacterial translocation (Lipopolysaccharide-binding protein or LBP) and systemic inflammation (IL-6) in 44 HIV virologically suppressed patients, half of whom (n = 22) had immunologic non-response to antiretroviral therapy (<270 CD4+Tcells/μL despite long-term suppressed viral load). The aim of the present study was to investigate if this beneficial effect of the probiotic Saccharomyces boulardii is due to modified gut microbiome composition, with a decrease of some species associated with higher systemic levels of microbial translocation and inflammation. In this study, we used 16S rDNA gene amplification and parallel sequencing to analyze the probiotic impact on the composition of the gut microbiome (faecal samples) in these 44 patients randomized to receive oral supplementation with probiotic or placebo for 12 weeks. Compared to the placebo group, in individuals treated with probiotic we observed lower concentrations of some gut species, such as those of the C lostridiaceae family, which were correlated with systemic levels of bacterial translocation and inflammation markers. In a sub-study of these patients, we observed significantly higher parameters of microbial translocation (LBP, soluble CD14) and systemic inflammation in immunologic non-responders than in immunologic responders, which was correlated with a relative abundance of specific gut bacterial groups (Lachnospiraceae genus and Proteobacteria). Thus, in this work, we propose a new therapeutic strategy using the probiotic yeast S. boulardii to modify gut microbiome composition. Identifying pro-inflammatory species in the gut microbiome could also be a useful new marker of poor immune response and a new therapeutic target.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	PloS one, Vol. 12 (april 2017) , ISSN 1932-6203

DOI: 10.1371/journal.pone.0173802
PMID: 28388647

15 p, 2.1 MB

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