Sputum Inflammatory Profile Before and After Specific Inhalation Challenge in Individuals with Suspected Occupational Asthma
Sánchez-Vidaurre, Sara (Universitat Autònoma de Barcelona. Departament de Medicina)
Cruz Carmona, María Jesús (Hospital Universitari Vall d'Hebron)
Gómez-Ollés, Susana (Hospital Universitari Vall d'Hebron)
Morell Brotad, Ferran 1944- (Hospital Universitari Vall d'Hebron)
Muñoz Gall, Xavier (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)

Data:	2013
Resum:	The aim of this study was to establish the sputum inflammatory profile and changes in levels of leukotriene B (LTB) and a panel of Th1/Th2 cytokines in subjects with suspected occupational asthma (OA) following specific inhalation challenge (SIC) to high-molecular-weight (HMW) and low-molecular-weight (LMW) agents. Fifty-one consecutive subjects undergoing SIC for suspected OA were enrolled. Sputum induction was performed the day before and 24 h after exposure to the offending agent. Total and differential cell counts were assessed. LTB and a 10 Th1/Th2 cytokines were measured in sputum supernatant. Thirty-four patients tested positive to SIC and were diagnosed with OA (in 10 due to HMW agents and in 24 to LMW agents). SIC was negative in 17 subjects. As compared to baseline an increase was found in the percentage of sputum eosinophils and neutrophils, and in IL-10 concentration after SIC (p = 0. 0078, p = 0. 0195, and p = 0. 046, respectively), and a decrease was seen in LTB level (p = 0. 0078) in patients with OA due to HMW agents. An increase in the percentage of sputum neutrophils after SIC (p = 0. 0040) was observed in subjects without OA exposed to LMW agents. IL-8 levels after SIC were higher in patients without OA compared with patients with OA (p = 0. 0146). When conducting airway inflammation studies in OA, patients should be divided according to the causal agent (HMW or LMW). In OA patients exposed to HMW agents, an increase in the number of neutrophils can be found in parallel to the increase of eosinophils, although this does not contradict an IgE-mediated mechanism. Exposure to LMW agents can result in increased neutrophilic inflammation in patients with airway diseases unrelated to OA. There is variability in the responses observed in patients with OA exposed to LMW agents.
Ajuts:	Ministerio de Economía y Competitividad CP12/03101 Instituto de Salud Carlos III FIS PI-05100
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	PloS one, Vol. 8, Issue 11 (November 2013) , art. e78304, ISSN 1932-6203

DOI: 10.1371/journal.pone.0078304
PMID: 24236015

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