Exogenous albumin is crucial for pig sperm to elicit in vitro capacitation whereas bicarbonate only modulates its efficiency
Resende Chaves, Bruna (Universitat de Girona. Institut de Tecnologia Agroalimentària)
Pavaneli, Ana Paula (University of São Paulo. Department of Animal Reproduction)
Blanco Prieto, Olga (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Pinart, Elisabeth (Universitat de Girona. Departament de Biologia)
Bonet, Sergi (Universitat de Girona. Departament de Biologia)
Zangeronimo, Marcio Gilberto (Federal University of Lavras, Brazil. Department of Veterinary Medicine)
Rodríguez Gil, Joan Enric (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Yeste Oliveras, Marc (Universitat de Girona. Departament de Biologia)

Fecha:	2021
Resumen:	This work sought to address whether the presence of exogenous bicarbonate is required for pig sperm to elicit in vitro capacitation and further progesterone-induced acrosome exocytosis. For this purpose, sperm were either incubated in a standard in vitro capacitation medium or a similar medium with different concentrations of bicarbonate (either 0 mM, 5 mM, 15 mM or 38 mM) and BSA (either 0 mg/mL or 5 mg/mL). The achievement of in vitro capacitation and progesterone-induced acrosomal exocytosis was tested through the analysis of sperm motility, plasma membrane integrity and lipid disorder, acrosome exocytosis, intracellular calcium levels, mitochondria membrane potential, O consumption rate and the activities of both glycogen synthase kinase 3 alpha (GSK3α) and protein kinase A (PKA). While sperm incubated in media without BSA or BSA/bicarbonate, they did not achieve in vitro capacitation; those incubated in media with BSA achieved the capacitated status under any bicarbonate concentration, even when bicarbonate was absent. Moreover, there were differences related to the concentration of bicarbonate, since sperm incubated in media with BSA and with no bicarbonate or 5 mM bicarbonate showed lower overall efficiency in achieving in vitro capacitation than those incubated in the presence of BSA and 15 mM or 38 mM bicarbonate. Additionally, at the end of the experiment, sperm incubated in the presence of BSA and 38 mM bicarbonate showed significantly (p < 0. 05) lower values of motility and plasma membrane integrity than those incubated in media with BSA and lower concentrations of bicarbonate. In conclusion, BSA is instrumental for pig sperm to elicit in vitro capacitation and trigger the subsequent progesterone-induced acrosome exocytosis. Furthermore, while exogenous bicarbonate does not seem to be essential to launch sperm capacitation, it does modulate its efficiency.
Ayudas:	Agència de Gestió d'Ajuts Universitaris i de Recerca 2017-SGR-1229 Ministerio de Ciencia, Innovación y Universidades AGL2017-88329-R Ministerio de Ciencia, Innovación y Universidades RYC-2014-15581
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Sperm capacitation ; Pig ; Bicarbonate ; Bovine serum albumin (BSA) ; Glycogen synthase kinase (GSK3) ; Protein kinase A (PKA)
Publicado en:	Biology, Vol. 10 Núm. 11 (november 2021) , p. 1105, ISSN 2079-7737

DOI: 10.3390/biology10111105
PMID: 34827098

24 p, 3.2 MB

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