Induced ligno-suberin vascular coating and tyramine-derived hydroxycinnamic acid amides restrict Ralstonia solanacearum colonization in resistant tomato
Kashyap, Anurag (Centre de Recerca en Agrigenòmica)
Jiménez Jiménez, Álvaro Luis (Centre de Recerca en Agrigenòmica)
Zhang, Weiqi (Centre de Recerca en Agrigenòmica)
Capellades, Montserrat (Centre de Recerca en Agrigenòmica)
Srinivasan, Sumithra (Institut de Ciència de Materials de Barcelona)
Laromaine, Anna (Institut de Ciència de Materials de Barcelona)
Serra, Olga (Universitat de Girona. Departament de Biologia)
Figueras, Mercè (Universitat de Girona. Departament de Biologia)
Rencoret, Jorge (Instituto de Recursos Naturales y Agrobiología de Sevilla)
Gutiérrez, Ana (Instituto de Recursos Naturales y Agrobiología de Sevilla)
Valls, Marc (Centre de Recerca en Agrigenòmica)
Sánchez Coll, Núria (Centre de Recerca en Agrigenòmica)

Date:	2022
Abstract:	Tomato varieties resistant to the bacterial wilt pathogen Ralstonia solanacearum have the ability to restrict bacterial movement in the plant. Inducible vascular cell wall reinforcements seem to play a key role in confining R. solanacearum into the xylem vasculature of resistant tomato. However, the type of compounds involved in such vascular physico-chemical barriers remain understudied, while being a key component of resistance. Here we use a combination of histological and live-imaging techniques, together with spectroscopy and gene expression analysis to understand the nature of R. solanacearum-induced formation of vascular coatings in resistant tomato. We describe that resistant tomato specifically responds to infection by assembling a vascular structural barrier formed by a ligno-suberin coating and tyramine-derived hydroxycinnamic acid amides. Further, we show that overexpressing genes of the ligno-suberin pathway in a commercial susceptible variety of tomato restricts R. solanacearum movement inside the plant and slows disease progression, enhancing resistance to the pathogen. We propose that the induced barrier in resistant plants does not only restrict the movement of the pathogen, but may also prevent cell wall degradation by the pathogen and confer anti-microbial properties, effectively contributing to resistance.
Grants:	Agencia Estatal de Investigación PID2019-110330GB-C21 Agencia Estatal de Investigación PID2020-118968RB-I00 Ministerio de Economía y Competitividad SEV-2015-0533 Agencia Estatal de Investigación CEX2019-000917 Agencia Estatal de Investigación CEX2019-000902-S Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-765 European Commission 754397
Note:	Altres ajuts: CERCA Programme/Generalitat de Catalunya i ajut del CSIC PIE-201620E081
Rights:	Tots els drets reservats.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	Bacterial wilt ; Feruloyltyramine ; HCAAs ; Lignin ; Ralstonia solanacearum ; Suberin ; Tomato ; Vascular coating
Published in:	The new phytologist, Vol. 234, Issue 4 (May 2022) , p. 1411-1429, ISSN 1469-8137

DOI: 10.1111/nph.17982

Postprint 60 p, 3.8 MB

Supplementary material 23 p, 2.8 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles