Evaluation of an O 2-Substituted (1-3)-β-D-Glucan, Produced by Pediococcus parvulus 2.6, in ex vivo Models of Crohn's Disease
Notararigo, Sara (Instituto de Investigación Sanitaria de Santiago (IDIS))
Varela, Encarnación (Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas)
Otal, Anna (Hospital Universitari Vall d'Hebron)
Cristobo, Iván (Centro de Investigaciones Biológicas (Madrid))
Antolín, María (Hospital Universitari Vall d'Hebron)
Guarner, Francisco (Hospital Universitari Vall d'Hebron)
Prieto, Alicia (Centro de Investigaciones Biológicas (Madrid))
López, Paloma (Centro de Investigaciones Biológicas (Madrid))
Universitat Autònoma de Barcelona

Date:	2021
Abstract:	1,3-β-glucans are extracellular polysaccharides synthesized by microorganisms and plants, with therapeutic potential. Among them, the O 2-substituted-(1-3)-β-D-glucan, synthesized by some lactic acid bacteria (LAB), has a prebiotic effect on probiotic strains, an immunomodulatory effect on monocyte-derived macrophages, and potentiates the ability of the producer strain to adhere to Caco-2 cells differentiated to enterocytes. In this work, the O 2-substituted-(1-3)-β-D-glucan polymers produced by GTF glycoyltransferase in the natural host Pediococcus parvulus 2. 6 and in the recombinant strain Lactococcus lactis NZ9000[pNGTF] were tested. Their immunomodulatory activity was investigated in an ex vivo model using human biopsies from patients affected by Crohn's disease (CD). Both polymers had an anti-inflammatory effect including, a reduction of Interleukine 8 both at the level of its gene expression and its secreted levels. The overall data indicate that the O 2-substituted-(1-3)-β-D-glucan have a potential role in ameliorating inflammation via the gut immune system cell modulation.
Grants:	Agencia Estatal de Investigación RTI2018-097114-B-I00 Ministerio de Economía y Competitividad PI12/00263
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Bacterial exopolysaccharides ; O2-substituted-(1-3)-β-D-glucan ; Lactic acid bacteria ; Immunomodulation ; Crohn's disease anti-inflammatory effect
Published in:	Frontiers in microbiology, Vol. 12 (february 2021) , ISSN 1664-302X

DOI: 10.3389/fmicb.2021.621280
PMID: 33613490

13 p, 1.5 MB

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