Utility of the Cobas ® Plasma Separation Card as a Sample Collection Device for Serological and Virological Diagnosis of Hepatitis C Virus Infection
Velásquez-Orozco, Fernando (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Rando-Segura, Ariadna (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Martínez-Camprecios, Joan (Hospital Universitari Vall d'Hebron)
Salmerón, Paula (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Najarro-Centeno, Adrián (Hospital Universitari Vall d'Hebron)
Esteban, Àngels (Hospital Universitari Vall d'Hebron)
Quer, Josep 1963- (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Buti, Maria (Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas)
Pumarola Suñé, Tomàs (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Rodríguez Frías, Francisco (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Date:	2021
Abstract:	Diagnosis and clinical management of people infected with hepatitis C virus (HCV) relies on results from a combination of serological and virological tests. The aim of this study was to compare the performance of dried plasma spots (DPS), prepared using the cobas ® Plasma Separation Card (PSC), to plasma and serum from venipuncture, for HCV diagnosis. We carried out a prospective study using DPS and paired plasma or serum samples. Serum and DPS samples were analyzed by immunoassay using Elecsys ® Anti-HCV II (Roche). Plasma and DPS samples were analyzed using the cobas ® HCV viral load and cobas ® HCV genotyping tests (Roche). All DPS samples that had high anti-HCV antibody titers in serum were also antibody-positive, as were five of eight samples with moderate titers. Eight samples with low titers in serum were negative with DPS. Among 80 samples with plasma HCV viral loads between 61. 5 and 2. 2 × 10 8 IU/mL, 74 were RNA-positive in DPS. The mean viral load difference between plasma and DPS was 2. 65 log IU/mL. The performance of DPS for detection of serological and virological markers of hepatitis C virus infection was comparable to that of the conventional specimen types. However, the limits of detection were higher for DPS.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Hepatitis C ; Dried plasma spot ; Plasma separation card
Published in:	Diagnostics, Vol. 11, Issue 3 (March 2021) , art. 473, ISSN 2075-4418

DOI: 10.3390/diagnostics11030473
PMID: 33800211

10 p, 1.5 MB

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